Team:Cambridge-JIC/Protocol

From 2014.igem.org

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<li> Pour the gel into two 50 ml falcon tubes </li>
<li> Pour the gel into two 50 ml falcon tubes </li>
<li> In a dedicated gel area, add 5 µl of Sybr Safe DNA dye to each falcon </li>
<li> In a dedicated gel area, add 5 µl of Sybr Safe DNA dye to each falcon </li>
 +
<li> Seal the sides of the gel mould with masking tape</li>
<li> Pour the gel from both falcon tubes into the gel mould and insert an 8 tooth comb</li>
<li> Pour the gel from both falcon tubes into the gel mould and insert an 8 tooth comb</li>
<li> Leave the gel to set for 30 mins </li>
<li> Leave the gel to set for 30 mins </li>

Revision as of 15:18, 31 July 2014