Team:Cambridge-JIC/Protocol

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<h3 id="Introduction">Introduction</h3>
<h3 id="Introduction">Introduction</h3>
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We are choosing to transform Marchantia by using agrobacteria to inject artificial plasmids into the Marchantia nuclei. A rough outline of the process is as follows:
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We transform Marchantia using agrobacteria. The agrobacteria inject artificial plasmids into the Marchantia nuclei. A rough outline of the process:
<ul>
<ul>
<li>Fragments of DNA are produced from a library of existing templates (plasmids)</li>
<li>Fragments of DNA are produced from a library of existing templates (plasmids)</li>
<li>These fragments are combined to produce the artificial plasmid</li>
<li>These fragments are combined to produce the artificial plasmid</li>
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<li>The artificial plasmid is amplified in E coli</li>
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<li>The artificial plasmid is amplified in E. coli</li>
<li>The artificial plasmid is incorporated into agrobacterium</li>
<li>The artificial plasmid is incorporated into agrobacterium</li>
<li>The agrobactria are used to infect Marchantia plants which are 5 days old</li>
<li>The agrobactria are used to infect Marchantia plants which are 5 days old</li>

Revision as of 13:29, 31 July 2014