Team:Warwick/Project

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            The Project
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            Meet the Team
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          <p class="title">Our Wiki is Under Development!</p>
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      <h1 class="title" align="center">The Project</h1>
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      <h2>The Problem</h2>
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        The Sheffield iGEM 2014 team have chosen to address the challenge of reducing the amount of fats, oils and greases (FOGs) entering the sewage system. These contribute to major costs and blockages in the systems known as <a href="http://tinyurl.com/mdhrfmk"><i>fatbergs</i> </a>. At present, there are over 200,000 major UK sewage blockages per year, of which <sup>3</sup>/<sub>4</sub> are caused by FOGs, meaning this is a major challenge to infrastructure and clearly a global challenge as cities expand, particularly in developing countries. These drain blockages in the UK are a growing problem costing Thames Water (London) alone, £1 million per month to tackle. In addition to FOGs, human hair is a well-known problem in wastewater treatment, being among a range of difficult to degrade materials.
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<a href="https://2014.igem.org/Team:Warwick"style="color:#000000"> <b> Home </b> </a> </td>
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      <h2>The Concept</h2>
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<a href="https://2014.igem.org/Team:Warwick/Team"style="color:#000000"> <b> Team </b> </a> </td>
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        Objectively, the team aim to build a genetically engineered, non-pathogenic strain of E. coli to responsively secrete lipase and keratinase in the presence of fats and hair in order to degrade and solubilise the components; this will ultimately prevent the blockages in the network. This will then be developed further in the future, by incorporating a transport system into the bacteria to uptake the lipid components to harvest and reuse as a potential energy source.
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      <h2>The Parts</h2>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Warwick"style="color:#000000"> <b> Official Team Profile </b> </a></td>
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        The system being created in E. coli is composed of several key BioBrick parts from the iGEM registry:
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<a href="https://2014.igem.org/Team:Warwick/Project"style="color:#000000"> <b> Project </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Parts"style="color:#000000"> <b> Parts </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Modeling"style="color:#000000"> <b> Modeling </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Notebook"style="color:#000000"> <b> Notebook </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Safety"style=" color:#000000"> <b> Safety </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Attributions"style="color:#000000"> <b> Attributions </b> </a></td>
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<a href="https://2014.igem.org/Team:Warwick/Measurements"style="color:#000000"> <b> Measurements </b> </a></td>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Warwick">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Warwick/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Warwick">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Warwick/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:Warwick/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:Warwick/Modeling">Modeling</a> </li>
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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            <th>iGEM Registry Part Code</th>
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            <th>BioBrick</th>
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            <th>Function</th>
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            <td>BBa_K258006</td>
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            <td>Thermostable lipase (TliA) of <i>P. fluorescens</i> SIK W1</td>
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            <td>The TliA lipase is secreted using an ABC transporter to degrade fats; it is thermostable and therefore efficient when functioning at high temperatures, such as within the water network.</td>
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            <td>BBa_K258008</td>
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            <td>ABC transporter of <i>E. chrysanthemi</i></td>
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            <td>A type I secretion system used to export proteins with C-terminal fragments, such as TliA</td>
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            <td>BBa_K861060</td>
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            <td>PfadR promoter</td>
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            <td>An artificial promoter containing a tandem FadR binding site which is not affected by glucose concentration, oxidative stress and several other factors.</td>
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            <td>BBa_K861061</td>
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            <td>RFP generator of PfadR</td>
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            <td>When placed downstream of K861060, efficacy of the promoter can be tested by allowing visualisation of red fluorescence protein.</td>
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            <td>BBa_K1149021</td>
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            <td>Composite used with BBa_K258008</td>
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            <td>This construct allows production of a promoter, RBS and lipase ABC transporter recognition domain to allow lipid secretion outside of the cell.</td>
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            <td>BBa_K215107</td>
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            <td>Lipase ABC transporter recognition domain</td>
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            <td>This domain will bind the lipase to allow secretion.</td>
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          <td>BBa_K215002</td>
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          <td>pLac + RBS + Secretion Signal and Streptavidin Binding Tags</td>
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          <td>This generator has a promoter which is IPTG-inducible and a strong RBS to allow expression of our transporter.</td>
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        Alongside these existing parts, we are developing our own keratinase enzyme part, which we will submit to the registry as a new, documented BioBrick.
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      </p>
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      <h2>The Approach</h2>
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      <p>
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        Alongside the laboratory work to construct this system, the team are collaborating with the water industry and potential users of the system in order to develop an implementation device for its application in society. The broader impact of this device will then be considered from many different aspects to evaluate the product. As well as this, outreach activities within and outside of the University to promote synthetic biology will be carrier out. These aim to deepen understanding of the new technology and gauge interest and support to allow successful incorporation of it into society.
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      <h1 class="title">Meet the Team</h1>
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            <div class="caption"><h1>Ben Madden</h1><p>Hi I'm Ben, a third year Chemical Engineer</p>
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            <a href="http://uk.linkedin.com/pub/ben-madden/84/38/40a" title="Find me on LinkedIn" alt="LinkedIn Icon" class="socialIcons"><img src="teamPageImages/linkedin grey.png" width="30px" height="30px"/></a>
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            <a href="mailto:brmaden1@sheffield.ac.uk" target="_top" title="Contact me via Email" alt="Email Icon" class="socialIcons"><img src="teamPageImages/email grey.png" width="30px" height="30px"/></a>
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            <div class="caption"><h1>Lara Grew</h1><p>Hi I'm Lara, a second year Geneticist</p>
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            <a href="mailto:lgrew1@sheffield.ac.uk" target="_top" title="Contact me via Email" alt="Email Icon" class="socialIcons"><img src="teamPageImages/email grey.png" width="30px" height="30px"/></a>
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            <div class="caption"><h1>Ben Lomax</h1><p>Hi I'm Ben, a first year MBB student</p>
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            <a href="mailto:blomax1@sheffield.ac.uk" target="_top" title="Contact me via Email" alt="Email Icon" class="socialIcons"><img src="teamPageImages/email grey.png" width="30px" height="30px"/></a>
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            <div class="caption"><h1>Erika Otaviano</h1><p>Hi I'm Erika, a third year BioEngineer</p>
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            <a href="mailto:eferreira1@sheffield.ac.uk" target="_top" title="Contact me via Email" alt="Email Icon" class="socialIcons"><img src="teamPageImages/email grey.png" width="30px" height="30px"/></a>
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            <div class="caption"><h1>Alex Simpson</h1><p>Hi I'm Alex, a second year Aerospace Engineer</p>
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            <div class="caption"><h1>Jianxing Qin</h1><p>Hi I'm Jay, a second year ACSE student</p>
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            <div class="caption"><h1>Mustafa Hussain</h1><p>Hi I'm Mustafa, a second year ACSE student</p>
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            <div class="caption"><h1>Sharan Nanuan</h1><p>Hi I'm Sharan, a second year MBB student</p>
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        ERASynBio promotes the robust development of Synthetic Biology by structuring and coordinating national efforts and investment. They have developed a strategic vision to support emergence of Synthetic Biology programmes to lay the ground for transnational funding activities via joint calls in the project. They stimulate and tackle the interdisciplinary nature and immaturity by offering training and educational possibilities, establishing an interdisciplinary advisory board and inviting observers of other funding organisations.
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        The Biotechnology and Biological Sciences Research Council (BBSRC) is a UK research council and non-departmental public body. It is the largest UK public funder of non-medical bioscience. It predominantly funds scientific research institures and university research departments in the UK.
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        The Biochemical Society is a learned society in the UK in the field of biochemistry, including all the cellular and molecular biosciences. It currently has approximately 7000 members, of which two-thirds are in the UK. The society’s headquarters are in London.
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        The Society for General Microbiology is a learned society based in the UK with a worldwide membership based in universities, industry, hospitals, research institutes and schools. Its headquarters are in Charles Darwin House, London. The society is a member of the Science Council.
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        The Wellcome Trust was established in 1936 as an independent charity funding research to improve human and animal health, as well as supporting the public understanding of science. It is the UK’s largest provider of non-government funding for science research and one of the largest providers in the world.
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        The Alumni Foundation at the University of Sheffield exists to channel donations from former students, staff and friends into projects involving current students and staff.
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        The University of Sheffield Enterprise brings people together to help make ideas happen by creating opportunities to develop potential projects.
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Revision as of 14:05, 31 July 2014

iGEM Sheffield 2014

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The Project

The Problem

The Sheffield iGEM 2014 team have chosen to address the challenge of reducing the amount of fats, oils and greases (FOGs) entering the sewage system. These contribute to major costs and blockages in the systems known as fatbergs . At present, there are over 200,000 major UK sewage blockages per year, of which 3/4 are caused by FOGs, meaning this is a major challenge to infrastructure and clearly a global challenge as cities expand, particularly in developing countries. These drain blockages in the UK are a growing problem costing Thames Water (London) alone, £1 million per month to tackle. In addition to FOGs, human hair is a well-known problem in wastewater treatment, being among a range of difficult to degrade materials.

The Concept

Objectively, the team aim to build a genetically engineered, non-pathogenic strain of E. coli to responsively secrete lipase and keratinase in the presence of fats and hair in order to degrade and solubilise the components; this will ultimately prevent the blockages in the network. This will then be developed further in the future, by incorporating a transport system into the bacteria to uptake the lipid components to harvest and reuse as a potential energy source.

The Parts

The system being created in E. coli is composed of several key BioBrick parts from the iGEM registry:

iGEM Registry Part Code BioBrick Function
BBa_K258006 Thermostable lipase (TliA) of P. fluorescens SIK W1 The TliA lipase is secreted using an ABC transporter to degrade fats; it is thermostable and therefore efficient when functioning at high temperatures, such as within the water network.
BBa_K258008 ABC transporter of E. chrysanthemi A type I secretion system used to export proteins with C-terminal fragments, such as TliA
BBa_K861060 PfadR promoter An artificial promoter containing a tandem FadR binding site which is not affected by glucose concentration, oxidative stress and several other factors.
BBa_K861061 RFP generator of PfadR When placed downstream of K861060, efficacy of the promoter can be tested by allowing visualisation of red fluorescence protein.
BBa_K1149021 Composite used with BBa_K258008 This construct allows production of a promoter, RBS and lipase ABC transporter recognition domain to allow lipid secretion outside of the cell.
BBa_K215107 Lipase ABC transporter recognition domain This domain will bind the lipase to allow secretion.
BBa_K215002 pLac + RBS + Secretion Signal and Streptavidin Binding Tags This generator has a promoter which is IPTG-inducible and a strong RBS to allow expression of our transporter.
Alongside these existing parts, we are developing our own keratinase enzyme part, which we will submit to the registry as a new, documented BioBrick.

The Approach

Alongside the laboratory work to construct this system, the team are collaborating with the water industry and potential users of the system in order to develop an implementation device for its application in society. The broader impact of this device will then be considered from many different aspects to evaluate the product. As well as this, outreach activities within and outside of the University to promote synthetic biology will be carrier out. These aim to deepen understanding of the new technology and gauge interest and support to allow successful incorporation of it into society.

×

Meet the Team

Ben Madden

Hi I'm Ben, a third year Chemical Engineer

Lara Grew

Hi I'm Lara, a second year Geneticist

Ben Lomax

Hi I'm Ben, a first year MBB student

Erika Otaviano

Hi I'm Erika, a third year BioEngineer

Alex Simpson

Hi I'm Alex, a second year Aerospace Engineer

Jianxing Qin

Hi I'm Jay, a second year ACSE student

Mustafa Hussain

Hi I'm Mustafa, a second year ACSE student

Sharan Nanuan

Hi I'm Sharan, a second year MBB student
×

Our Sponsors

ERASynBio promotes the robust development of Synthetic Biology by structuring and coordinating national efforts and investment. They have developed a strategic vision to support emergence of Synthetic Biology programmes to lay the ground for transnational funding activities via joint calls in the project. They stimulate and tackle the interdisciplinary nature and immaturity by offering training and educational possibilities, establishing an interdisciplinary advisory board and inviting observers of other funding organisations.

The Biotechnology and Biological Sciences Research Council (BBSRC) is a UK research council and non-departmental public body. It is the largest UK public funder of non-medical bioscience. It predominantly funds scientific research institures and university research departments in the UK.

The Biochemical Society is a learned society in the UK in the field of biochemistry, including all the cellular and molecular biosciences. It currently has approximately 7000 members, of which two-thirds are in the UK. The society’s headquarters are in London.

The Society for General Microbiology is a learned society based in the UK with a worldwide membership based in universities, industry, hospitals, research institutes and schools. Its headquarters are in Charles Darwin House, London. The society is a member of the Science Council.

The Wellcome Trust was established in 1936 as an independent charity funding research to improve human and animal health, as well as supporting the public understanding of science. It is the UK’s largest provider of non-government funding for science research and one of the largest providers in the world.

The Alumni Foundation at the University of Sheffield exists to channel donations from former students, staff and friends into projects involving current students and staff.

The University of Sheffield Enterprise brings people together to help make ideas happen by creating opportunities to develop potential projects.

×

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