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Team:Caltech/week9
From 2014.igem.org
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<b>agrBCDA Reception System and Combinatorial Promoters</b> | <b>agrBCDA Reception System and Combinatorial Promoters</b> | ||
| - | <ul> | + | agrBCDA system: |
| + | <ul><li>Set up liquid cultures of pAA009 & pAA010 colonies from overnight.</li> | ||
| + | <li>Ran colony PCR on pAA009 & pAA010 colonies, adding DMSO & with annealing temperature of 51°C. This yielded bright bands at correct length for 2 of the pAA010 colonies and some of the pAA009 colonies.</li> | ||
| + | <li>PCR-purified the pAA010 colony PCR products & shipped them for sequencing.</li> | ||
| + | <li>Miniprepped pAA009 liquid cultures & sent samples for sequencing.</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
Revision as of 04:39, 12 August 2014
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