Week 2

Monday

Lab meeting

• in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
• monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
• tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
• tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A

@YK need to email brian! what we need for lab space when we move

Edo

Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed.

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Adam

Kevin

Dave

Lewis B

Rob

Myriam

Alberto

Tuesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Wednesday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Thursday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Friday

Lewis M

Sanjay

Maurice

Joy

Ning

Pamela

Bez

Daniel

Sohaila

Georgia

Yan-Kay

Tanel

Weekend