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Team:Paris Saclay/Protocols/PCR clean-up
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= '''PCR clean-up''' = | = '''PCR clean-up''' = | ||
| + | |||
| + | After the PCR, pool your PCR result in an eppendorf 1,5ml. | ||
| + | Add 200µl NTI. | ||
| + | |||
| + | Centrifuge at 11000g, 30 secondes RT. | ||
| + | Discard the supernatant, wash first time with 700µl NT3. | ||
| + | Centrifuge at 11000g, 30 secondes RT. | ||
| + | Discard the supernatant, wash a second time with 700µl NT3. | ||
| + | Centrifuge at 11000g, 30 secondes RT. | ||
| + | |||
| + | Centrifuge the column empty. | ||
| + | |||
| + | Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. | ||
| + | Collect your DNA in an eppendorf 1,5ml. | ||
Revision as of 13:01, 25 July 2014
PCR clean-up
After the PCR, pool your PCR result in an eppendorf 1,5ml. Add 200µl NTI.
Centrifuge at 11000g, 30 secondes RT. Discard the supernatant, wash first time with 700µl NT3. Centrifuge at 11000g, 30 secondes RT. Discard the supernatant, wash a second time with 700µl NT3. Centrifuge at 11000g, 30 secondes RT.
Centrifuge the column empty.
Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. Collect your DNA in an eppendorf 1,5ml.
