Team:Paris Saclay/Notebook/July/24

From 2014.igem.org

(Difference between revisions)
(→Thursday 24th July)
(→Thursday 24th July)
Line 10: Line 10:
Biobricks used:
Biobricks used:
-
*BBa_K1033927 (pink chromoprotein)
+
*BBa_K1033927 with pSB1C3 (pink chromoprotein)
-
*BBa_K1033910 (yellow chromoprotein)
+
*BBa_K1033910 with pSB1C3 (yellow chromoprotein)
-
*BBa_K1033921 (red chromoprotein)
+
*BBa_K1033921 with pSB1C3 (red chromoprotein)
-
*BBa_K1033922 (red chromoprotein)
+
*BBa_K1033922 with pSB1C3 (red chromoprotein)
-
*BBa_K1033902 (blue chromoprotein)
+
*BBa_K1033902 with pSB1C3 (blue chromoprotein)
-
*BBa_K731201 (Arabinose inductible araC-pBAD promoter)
+
*BBa_K731201 with pSB1C3 (Arabinose inductible araC-pBAD promoter)
-
*BBa_K1033925 (pink chromoprotein)
+
*BBa_K1033925 with pSB1C3 (pink chromoprotein)
-
*BBa_K1033905 (purple chromoprotein)
+
*BBa_K1033905 with pSB1C3 (purple chromoprotein)
-
*BBa_K1033913 (orange chromoprotein)
+
*BBa_K1033913 with pSB1C3 (orange chromoprotein)
-
*BBa_K1033929 (blue chromoprotein)
+
*BBa_K1033929 with pSB1C3 (blue chromoprotein)
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
Line 30: Line 30:
Biobricks used:
Biobricks used:
-
*BBa_K762100 (Limonen synthase1 with RBS)
+
*BBa_K762100 with pSB1C3 (Limonen synthase1 with RBS)
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
Line 67: Line 67:
Biobricks used:
Biobricks used:
-
*BBa_J45017 (pchBA synthase (converts chorismate to salicylate))
+
*BBa_J45017 with pSB1C3 with pSB1AK3 (pchBA synthase (converts chorismate to salicylate))
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]

Revision as of 08:42, 25 July 2014

Contents

Thursday 24th July

Lab work

B - Construction of the fusion protein (color)

Plasmid DNA Extraction

by Terry

Biobricks used:

  • BBa_K1033927 with pSB1C3 (pink chromoprotein)
  • BBa_K1033910 with pSB1C3 (yellow chromoprotein)
  • BBa_K1033921 with pSB1C3 (red chromoprotein)
  • BBa_K1033922 with pSB1C3 (red chromoprotein)
  • BBa_K1033902 with pSB1C3 (blue chromoprotein)
  • BBa_K731201 with pSB1C3 (Arabinose inductible araC-pBAD promoter)
  • BBa_K1033925 with pSB1C3 (pink chromoprotein)
  • BBa_K1033905 with pSB1C3 (purple chromoprotein)
  • BBa_K1033913 with pSB1C3 (orange chromoprotein)
  • BBa_K1033929 with pSB1C3 (blue chromoprotein)

Protocol

C - Lemon scent

Plasmid DNA Extraction

by Terry

Biobricks used:

  • BBa_K762100 with pSB1C3 (Limonen synthase1 with RBS)

Protocol

Gel electrophoresis of p cola

LU000081.jpg

by Sean

p cola used: sample left overnight yesterday resuspended in 30μl of H2O milliQ.

Protocol

Results

Results of the PCR Targeting

by Romain

PCR targeting made the July 22nd.

Results:

  • Nothing has grown on the control dishes.
  • Failure, nothing has grown on the clone I dishes.
  • Failure, other bacteria have grown on the clone II dishes.

We have to make another PCR.

E - The Suppressing Salicylate System

Plasmid DNA Extraction

by Terry

Biobricks used:

  • BBa_J45017 with pSB1C3 with pSB1AK3 (pchBA synthase (converts chorismate to salicylate))

Protocol

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).

Samples:

  1. BBa_J61051 Concentrated
  2. BBa_J61051 Undiluted
  3. BBa_K228001 Concentrated
  4. BBa_K228001 Undiluted
  5. DH5α Control

Protocol

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/24"