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Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP
From 2014.igem.org
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<h4> Aim of the construct </h4> | <h4> Aim of the construct </h4> | ||
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| - | <li> | + | <li> We'll try out 35s->GAL4->HAP1GR->H2BmRFP. </li> |
| + | <li> Since H2BmRFP has not been tried in Mp, we'll also try 35s->H2BmRFP </li> | ||
| + | |||
</ul> | </ul> | ||
Revision as of 14:35, 24 July 2014
Gal4 - Hap1GR - H2BmRFP
Design
Aim of the construct
- We'll try out 35s->GAL4->HAP1GR->H2BmRFP.
- Since H2BmRFP has not been tried in Mp, we'll also try 35s->H2BmRFP
End goal plasmid
Experimentation (up to date)
PCR
Attempt 1:- Start date/time: 24/07, 11:08
- End date/time:24/07, 1550
- UIDs of primers/plasmids used:
- A1, P33, P34
- A1, P35, P36
- A2, P37, P38
- A2, P39, P40
- PCR Spreadsheet (on google drive)
- Gel 27/07/2014
Gibson
Attempt 1:- Start date/time:
- End date/time:
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E-Coli transformation
Attempt 1:- Start date/time:
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Agrobacteria transformation
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- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
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Spore transformation
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Evaluation
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