Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

(Difference between revisions)
Line 36: Line 36:
<li> A2, P39, P40 </ul></li>
<li> A2, P39, P40 </ul></li>
-
<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li>
+
<li><a href="4.https://docs.google.com/spreadsheets/d/1RwA7YHMnZ4a0M67RMbegWU5ee3MJRo62qOcBJj537yg/edit#gid=1660015451">PCR Spreadsheet (on google drive)</a></li>
<li><a href="http://google.com/GEL_27_07_2014">Gel 27/07/2014</a></li>
<li><a href="http://google.com/GEL_27_07_2014">Gel 27/07/2014</a></li>
</ul>
</ul>

Revision as of 14:30, 24 July 2014

Gal4 - Hap1GR - H2BmRFP

Design

Aim of the construct

  • Describe aims here

End goal plasmid

Experimentation (up to date)

PCR

Attempt 1:

Gibson

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

E-Coli transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Agrobacteria transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Induce comments:
  • Growth comments:
  • Electroporation/selection comments:

Spore preparation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Spore transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Evaluation

  • Start date/time:
  • End date/time:
  • Comments:

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