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Team:Cambridge-JIC/Notebook/CL W4 Wednesday
From 2014.igem.org
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Looking at codon table: c preferred at the end of codons and c-g sites frequent, as in Arabidopsis.<br> | Looking at codon table: c preferred at the end of codons and c-g sites frequent, as in Arabidopsis.<br> | ||
Wrote up on the bioinformatics progress so far.<br> | Wrote up on the bioinformatics progress so far.<br> | ||
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| + | <h5> Will </h5> | ||
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| + | Enhancer trap experimental design. Vector for random integration of minimal promoter into Marchy genome designed, and primers acquired. Jim gave me his vector for amplification of the minimal promoter (NB: it's an Arabidopsis promoter- although surely there must be some degree of homology between Eukaryotas, and *definitely* among plants). Ideally need Bernardo's vector as well. Primers arrive on Friday; will make vector then. | ||
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Revision as of 09:37, 24 July 2014
Ginny's day
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escaped catastrophe: primers made yesterday were pretty wrong...
remade new set_phew. MoClo_Biobrick compatible.
wetwork: co-cultured Marchantia spores with Agoro (RFP & GFP)
pursued secret side experiment___hold your breath
Trang-Anh's day
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Got the codon counter to work (yay!) and ran the analysis on the monster. Obtained marchy codon table.
Looking at codon table: c preferred at the end of codons and c-g sites frequent, as in Arabidopsis.
Wrote up on the bioinformatics progress so far.
Will
-
Enhancer trap experimental design. Vector for random integration of minimal promoter into Marchy genome designed, and primers acquired. Jim gave me his vector for amplification of the minimal promoter (NB: it's an Arabidopsis promoter- although surely there must be some degree of homology between Eukaryotas, and *definitely* among plants). Ideally need Bernardo's vector as well. Primers arrive on Friday; will make vector then.
