"
Team:Paris Saclay/Notebook/July/22
From 2014.igem.org
(→C - Lemon scent) |
(→C - Lemon scent) |
||
| Line 68: | Line 68: | ||
<span style="color:red">Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.</span> | <span style="color:red">Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.</span> | ||
| - | ==C - Lemon scent== | + | ==<span style="color:green">C - Lemon scent</span>== |
===PCR Targeting=== | ===PCR Targeting=== | ||
Revision as of 20:09, 23 July 2014
Contents |
Tuesday 22st July
Lab Work
1 - Samples for stock
by Fabio
We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.
- BBa_K1033902
- BBa_K1033905
- BBa_K1033910
- BBa_K1033913
- BBa_K1033922
- BBa_K1033925
- BBa_K1033927
- BBa_K1033929
- BBa_K103921
- BBa_J45017
- BBa_K731201
- BBa_K762100
from Liquide Bacterial Cultures transformed the 21st July
2 - Electrophoresis
by Arnaud (Process B), Fabio (gel), Marie and Romain (Process B)
We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.
Process A
Process B
Results:
- A:
- B:
- C:
3 - Bacterial Culture
by Fabio and Marie
One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.
4 - Plasmid DNA extraction
by Sean and Terry
Plasmids used: cf part 1
Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.
C - Lemon scent
PCR Targeting
by Romain
Strains used: DY330
Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)
Members there:
- Instructors and advisors: Solenne.
- Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
