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Team:Paris Saclay/Notebook/July/21
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| - | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 | + | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl1] |
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| - | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 | + | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23100 Cl2] |
| - | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 | + | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl1] |
| - | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 | + | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl2] |
| - | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 | + | #[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23114 Cl1] |
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'''''Process C''''' | '''''Process C''''' | ||
Revision as of 15:42, 23 July 2014
Contents |
Monday 21st July
Lab Work
1 - Results: Transformation of CaCl2 supercompetent cells
by Romain
Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures transformed the 18th July
Results: Nothing has grown.
2 - Results: Transformation of DY330 via pJBEI6409
by Sean
Transformation performed on the 18th July
| Volume of plasmid used | 50μl from cuvette | 100μl | remainder (850µl) |
|---|---|---|---|
| 2μl of plasmid | 0 | 4 | 30 |
| 4μl | 3 | 6 | 45 |
| Control | 0 | 0 | 0 |
Conclusion: The increase in colony number is proportional to the increase in volume used. The control yielded nothing. The results are coherent.
3 - Oligonucleotide's design
by Romain
For the banana scent, design of iPS91 and iPS92, the ilvE sequence to transformed the Leucin into α-cetoisocaproate (The first step).
4 - Liquid Bacterial Culture
by Marie, Romain & Sean
- DY330 pJBEI6409 with 10µl Cm in 10ml LB (x2)
- BT340 Cm and Amp
- The new strains received
5 - Electrophoresis
by Fabio (process A) and Mathieu (process B and C)
We used 2µl of DNA of the following Biobricks in a 1% Agarose Gel. The PCRs came from Mathias' manipulation made the 18th July.
Process A
- BBa_J23119 Cl1
- BBa_J23119 Cl2
- BBa_J23106 Cl1
- BBa_J23100 Cl2
- PCR 1
- PCR 2
- PCR 3
- PCR 4
- PCR 5
- PCR 6
- PCR 7
- PCR 8
- PCR 9
- PCR 10
Process B
Process C
Pooling and purifying PCR 9 and 10 from process A.
- PCR purified products. IPS70 / IPS71 of pOsV230 (Apramycin)
- BT 340 (plasmid's flipase)
Results:
- A: From 1 to 4: Success, DNAs have the expected size.
- A: From 5 to 12: Failure, No PCR products.
- A: Numbers 13 and 14: Success, PCR products have the expected size.
- B: All 6 extractions were successful.
- C: Number 1: successful concentration of pOsV230's PCR product.
- C: Number 2 had no migration.
People there:
- Instructors and advisors: Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
