Team:Oxford/protocols/Frozen Cell Stock Preparation

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<h1>Competent Cell Preparation</h1>
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<h1>Frozen Cell Stock Preparation</h1>

Revision as of 15:05, 4 August 2014



Frozen Cell Stock Preparation



    ↩ Back to other protocols.


  1. Grow a liquid cell culture of the strain to be made competent overnight.

  2. Pipette 0.666ml of liquid cell culture with 0.333ml of 60% glycerol (to give an overall percentage glycerol 20% and total volume of 1ml) into an eppendorf tube.

  3. Put into the freezer at -80°C.

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