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Team:Oxford/InterlabDevices
From 2014.igem.org
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We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent E.coli cells (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for deivces 2a, 2b/3b, and 3a) using the <html><a href="https://2014.igem.org/Team:Oxford/protocols/Transformation_into_chemically_competent_E.coli">standard E.coli transformation protocol</a></html> (however, we used 200 uL of competent cells per transformation rather than 100uL). | We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent E.coli cells (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for deivces 2a, 2b/3b, and 3a) using the <html><a href="https://2014.igem.org/Team:Oxford/protocols/Transformation_into_chemically_competent_E.coli">standard E.coli transformation protocol</a></html> (however, we used 200 uL of competent cells per transformation rather than 100uL). | ||
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| + | <p><font style="font-weight:bold">II. Growing liquid cultures:</font></p> | ||
| + | The next day, we have collected our plates from the 37°C incubator. All plates had colonies growing on them and we took one of each for generating <html><a href="https://2014.igem.org/Team:Oxford/protocols/Growing_Liquid_Cell_Cultures">liquid cultures</a></html>. | ||
Revision as of 12:24, 24 July 2014
Devices
I. Using the DNA distribution kit to extract devices 1-3:
We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent E.coli cells (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for deivces 2a, 2b/3b, and 3a) using the standard E.coli transformation protocol (however, we used 200 uL of competent cells per transformation rather than 100uL).
II. Growing liquid cultures:
The next day, we have collected our plates from the 37°C incubator. All plates had colonies growing on them and we took one of each for generating liquid cultures.
