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Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP
From 2014.igem.org
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Revision as of 14:48, 23 July 2014
Gal4 - Hap1GR - H2BmRFP
Design
Aim of the construct
- Describe aims here
End goal plasmid
Experimentation (up to date)
PCR
Attempt 1:- Start date/time:
- End date/time:
- UIDs of primers/plasmids used:
- A2, P12, P13
- B3, P14, P13
- A4, P12, P13
- PCR Spreadsheet (on google drive)
- Gel 27/07/2014
Gibson
Attempt 1:- Start date/time:
- End date/time:
- Comments:
E-Coli transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Agrobacteria transformation
Attempt 1:- Start date/time:
- End date/time:
- Induce comments:
- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Spore transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Evaluation
- Start date/time:
- End date/time:
- Comments:
