Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

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<h4> End goal plasmid </h4>
<h4> End goal plasmid </h4>
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<li> <img src="https://2014.igem.org/File:Cambridge-JIC_constructs_GAL4-HAP1GR-H2mRFP.png"> </li>
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Revision as of 14:48, 23 July 2014

Gal4 - Hap1GR - H2BmRFP

Design

Aim of the construct

  • Describe aims here

End goal plasmid

Experimentation (up to date)

PCR

Attempt 1:

Gibson

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

E-Coli transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Agrobacteria transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Induce comments:
  • Growth comments:
  • Electroporation/selection comments:

Spore preparation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Spore transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Evaluation

  • Start date/time:
  • End date/time:
  • Comments: