Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP

From 2014.igem.org

(Difference between revisions)
Line 30: Line 30:
<li>End date/time:</li>
<li>End date/time:</li>
<li> UIDs of primers/plasmids used:  
<li> UIDs of primers/plasmids used:  
 +
<ul>
<li> A2, P12, P13</li>
<li> A2, P12, P13</li>
<li> B3, P14, P13</li>
<li> B3, P14, P13</li>
-
<li> A4, P12, P13 </li>
+
<li> A4, P12, P13 </ul></li>
<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li>
<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li>

Revision as of 18:38, 22 July 2014

Gal4 - Hap1GR - H2BmRFP

Design

Aim of the construct

  • Describe aims here

End goal plasmid

  • insert image here

Experimentation (up to date)

PCR

Attempt 1:

Gibson

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

E-Coli transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Agrobacteria transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Induce comments:
  • Growth comments:
  • Electroporation/selection comments:

Spore preparation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Spore transformation

Attempt 1:
  • Start date/time:
  • End date/time:
  • Comments:

Evaluation

  • Start date/time:
  • End date/time:
  • Comments:

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