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Team:Paris Saclay/Notebook/July/16
From 2014.igem.org
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We transformed in DH5α the following Biobrick: | We transformed in DH5α the following Biobrick: | ||
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| - | * | + | *BBa_J23100 (x2) |
| - | * | + | *BBa_J23106 (x2) |
| - | * | + | *BBa_J23114 (x2) |
from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July] | from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July] | ||
Revision as of 15:38, 23 July 2014
Contents |
Wednesday 16th July
Lab work
1 - Transformation in DH5α
by Fabio, Maher and Romain
We transformed in DH5α the following Biobrick:
- BBa_J23119
Samples:
- Concentrated
- Undiluted
- Diluted 10-1
- Control
Results:
- Concentrated: 99 colonies
- Undiluted: 5 colonies
- Diluted: 0
- Control: 0
Conclusion:
Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
2 - Plasmid DNA Purification
by Mathieu
- BBa_J23100 (x2)
- BBa_J23106 (x2)
- BBa_J23114 (x2)
from Bacterial Culture made the 15th July
Reunion
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Reunion: Lab meeting to discuss the project. (afternoon)
Meeting with Evry's Team. (night) Arnaud represents us
