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Team:Oxford/protocols/Frozen Cell Stock Preparation
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Revision as of 13:29, 23 July 2014
Competent Cell Preparation
- Grow a liquid cell culture of the strain to be made competent overnight.
- Pipette 0.666ml of liquid cell culture with 0.333ml of 60% glycerol (to give an overall percentage glycerol 20% and total volume of 1ml) into an eppendorf tube.
- Put into the freezer at -80°C.
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