"
Team:Paris Saclay/Notebook/July/17
From 2014.igem.org
(Difference between revisions)
(→2 - Bacterial Culture) |
(→Thursday 17th July) |
||
| Line 22: | Line 22: | ||
''by Arnaud & Mathias'' | ''by Arnaud & Mathias'' | ||
| - | PCR targeting with oligos IPS70 and IPS71. | + | PCR targeting with oligos IPS70 and IPS71 of pOsV230 (Apramycine). |
#Molecular weight marker 1kb | #Molecular weight marker 1kb | ||
| Line 28: | Line 28: | ||
#IPS70 and IPS71 (1) | #IPS70 and IPS71 (1) | ||
#IPS70 and IPS71 (2) | #IPS70 and IPS71 (2) | ||
| + | |||
| + | '''Results:''' | ||
| + | |||
| + | Failure, no PCR products. | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | ||
| - | People there: | + | '''People there:''' |
* Instructors and advisors: Alice and Solenne. | * Instructors and advisors: Alice and Solenne. | ||
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 16:19, 21 July 2014
Contents |
Thursday 17th July
Lab work
1 - Preparation of supercompetent cells
By Mathias & Romain
Strains used: MG1655 (x2), MG1655Z1 (x2).
2 - Bacterial Culture
by Fabio and Maher
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both J23119 from the concentrated colony made the 16th July
3 - PCR and Electrophoresis
by Arnaud & Mathias
PCR targeting with oligos IPS70 and IPS71 of pOsV230 (Apramycine).
- Molecular weight marker 1kb
- Empty
- IPS70 and IPS71 (1)
- IPS70 and IPS71 (2)
Results:
Failure, no PCR products.
People there:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
