Team:Cambridge-JIC/Protocol

From 2014.igem.org

(Difference between revisions)
Line 2: Line 2:
<html>
<html>
 +
 +
 +
General outline of the method:
 +
<ol>
 +
<li> Polymerase Chain Reaction (PCR) to obtain DNA fragments </li>
 +
<li> Gel electrophoresis to select the correct fragments </li>
 +
<li> DNA purification for plasmid assembly </li>
 +
<li> Gibson Assembly </li>
 +
<li> E. coli transformation and plating </li>
 +
<li> Colony analysis and selection by colony PCR </li>
 +
<li> Miniprep and sequencing </li>
 +
</ol>
 +
</html>
</html>

Revision as of 09:21, 21 July 2014

Home Team Official Team Profile TimeLine Project Outreach Marchantia Informatics Parts Modeling Notebook Protocols Safety Dry Work Technology Attributions

General outline of the method:

  1. Polymerase Chain Reaction (PCR) to obtain DNA fragments
  2. Gel electrophoresis to select the correct fragments
  3. DNA purification for plasmid assembly
  4. Gibson Assembly
  5. E. coli transformation and plating
  6. Colony analysis and selection by colony PCR
  7. Miniprep and sequencing

Retrieved from "http://2014.igem.org/Team:Cambridge-JIC/Protocol"