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Team:BNU-China/home.html
From 2014.igem.org
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| - | Mr <i>E.coli Prometheus</i> is a philanthropic | + | Mr <i>E.coli Prometheus</i> is a philanthropic bacterium. One day, he saw many poor peanuts living in so tough a condition that they couldn't grow up nor to flower. He was determined to do something and help! </div> |
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/6/60/Bnu_s3.jpg" alt="3" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/6/60/Bnu_s3.jpg" alt="3" /> </div> | ||
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| - | + | He turned to us. He asked us for the power to collect molybdate and send them to peanut. We told him: " Since you were not naturally born with the power, for the sake of other lives, you must die after this." But he was as determined as ever.</div> | |
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/1/11/Bnu_s4.jpg" alt="4" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/1/11/Bnu_s4.jpg" alt="4" /> </div> | ||
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| - | <i>E.coli Prometheus</i> collected | + | With his power, <i>E.coli Prometheus</i> collected molybdate ...</div> |
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/9/9c/Bnu_s5.jpg" alt="5" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/9/9c/Bnu_s5.jpg" alt="5" /> </div> | ||
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| - | He sent the precious | + | He sent the precious micro-nutrient to those peanut everyday. The peanuts became more and more healthier and was about to flower ... |
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Revision as of 03:17, 18 October 2014
Maps
Our work
The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots.
We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.
Wiki Design
Art design, pictures, figures and the latest experiment results were put on the wiki, an important platform to show our works.
ModA & INPN
ModA we use in our project can bind the molybdate, and INPN can display ModA on E.coli surface.
Chemotaxis
We use McfR plasmid to give E.coli the capacity of Chemotaxis, so that those E.coli carrying molybdate can "swim" to the root of peanut.
Modeling
We have made a model of peanut roots and E.coli in surrounding environment to simulate our idea in soil.
Latest Works
Our Sponsors
The Story of E.coli Prometheus
BNU-China
