Team:Paris Bettencourt/Results

From 2014.igem.org

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<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></h6><br>
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab study</a></h6><br>
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<p class=text1>The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.</br>iGEM Paris Bettencourt team
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<li>Successfully built the three devices</li>
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                      <ul class=liste><li>- Successfully transformed <i>E.coli</i> NEB  with <b>BBa_I20260</b> (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - <b>Device 1</b></li>
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<li>Sequenced and gel verified the three devices</li>
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                      <li>- Successfully built <b>BBa_J23101 + BBa_E0240</b> (B0032-E0040-B0015) in pSB1C3 backbone - <b>Device 2</b></li>           
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<li>Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</li><li>Reported the results on the <a href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</li>
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                      <li>- Successfully built <b>BBa_J23115 + BBa_E0240</b> (B0032-E0040-B0015) in pSB1C3 backbone - <b>Device 3</b></li>
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<li>Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (Device 1)
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                      <li>- Sequenced and gel verified the three devices</li>
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<li>Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> (Device 2) and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> (Device 3) to the Biobricks registry</li>  
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                      <li>- Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader</li>
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                      <li>- Reported the results on the <a href'="https://2014.igem.org/Team:Paris_Bettencourt/Project/Interlab_Study">Interlab Study</a> page of the iGEM wiki</li>
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                      <li>- Completed the description of the <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> - <b>Device 1</b></li>
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                      <li>- Submitted and send parts: <a href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> -<b> Device 2</b> and <a href="http://parts.igem.org/Part:BBa_K1403001">BBa_K1403001</a> - <b>Device 3</b> to the Biobricks registry</li>  
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                       </ul>
                       </ul>
                 </p>
                 </p>

Revision as of 02:33, 18 October 2014

Smell
the roses		 Teen
spirit		 Something
fishy		 Goody
two shoes		 Don't
sweat it



Smell The Roses

  • Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.
  • We produced the smells that compose the main odor categories perceived by humans.
  • BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019

    • Teen Spirit

    ‘

    • Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
    • Determined the critical smell detection limit of 2-nonenal.
    • Analyzed 2-nonenal tolerance and degradation in natural isolates.

    Something Fishy

    • Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
    • Characterized and quantified the activity of TMM by a colorimetric assay.
    • Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
    .

    Goody Two Shoes

  • Demonstrated that mutant strains of B. subtilis produce less odor.
  • Measured growth and viability of mutants in synthetic sweat media and on socks.

    • Don't Sweat It

  • Expressed the odor gene agaA in E. coli (BBa_K1403002).
  • Designed and used CRISPRs to enrich for loss-of-function mutants 1000x in a mixed population.
  • Identified potential CRISPR targets as odor genes with variability hotspots in metagenome data.
  • Formulated a simple skin cream encorporating live bacteria.

    • Interlab study

  • Successfully built the three devices
  • Sequenced and gel verified the three devices
  • Characterised all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
  • Reported the results on the Interlab Study page of the iGEM wiki
  • Completed the description of the BBa_I20260 (Device 1)
  • Submitted and send parts: BBa_K1403000 (Device 2) and BBa_K1403001 (Device 3) to the Biobricks registry

    • Collaborations

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    Centre for Research and Interdisciplinarity (CRI)
    Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
    Paris Descartes University
    24, rue du Faubourg Saint Jacques
    75014 Paris, France
    +33 1 44 41 25 22/25
    paris-bettencourt-igem@googlegroups.com
    Copyright (c) 2014 igem.org. All rights reserved.

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