"
Team:Paris Bettencourt/Results
From 2014.igem.org
HenryDeBelly (Talk | contribs) |
HenryDeBelly (Talk | contribs) |
||
| Line 124: | Line 124: | ||
<p class=text2></p> | <p class=text2></p> | ||
<h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></h6><br> | <h6><a class=link href="https://2014.igem.org/Team:Paris_Bettencourt/Project/Odor_Library">Smell The Roses</a></h6><br> | ||
| - | <p class=text1> | + | <p class=text1><li>Created BioBricks coding for sequences for different enzymes to nullify the bad odor produced by E. coli.</li> |
| + | <li>We produced the smells that compose the main odor categories perceived by humans.</li> | ||
| + | <li>BioBricks submitted to be BioBrick registry:BBa_K1403003, BBa_K1403006, BBa_K1403009, BBa_K1403012, BBa_K1403017, BBa_K1403019 </li></p> | ||
</div> | </div> | ||
<div class=project id=project2> | <div class=project id=project2> | ||
Revision as of 02:29, 18 October 2014
| Smell the roses |
Teen spirit |
Something fishy |
Goody two shoes |
Don't sweat it |
Smell The Roses
Teen Spirit
‘
- Isolated 4 bacterial species capable of growing on 2-nonenal as a carbon source.
- Determined the critical smell detection limit of 2-nonenal.
- Analyzed 2-nonenal tolerance and degradation in natural isolates.
Something Fishy
- Cloned tmm into E.coli using pSB1C3 and pSEVA315 vectors, creating a new Biobrick: BBa_K1403015.
- Characterized and quantified the activity of TMM by a colorimetric assay.
- Confirmed the degradation of trimethylamine by gas chromatography - mass spectrometry (GC/MS).
Goody Two Shoes
Don't Sweat It
- Designed a BioBrick of agaA (main gene responsible for body odor) in pSB1C3.
- Found hotspots for genes ackA and ldh (responsible for body odor) found in human microbiome samples.
- Design CRISPRs to select for natural odorless strains.
- Made a DIY formulation of probiotic cream.
Interlab study
The aim of the Interlab Study was to measure GFP expression under different Anderson promoters and in different plasmids.iGEM Paris Bettencourt team
- - Successfully transformed E.coli NEB with BBa_I20260 (J23101-B0032-E0040-B0010-B0012) in the pSB3K3 vector - Device 1
- - Successfully built BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 2
- - Successfully built BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) in pSB1C3 backbone - Device 3
- - Sequenced and gel verified the three devices
- - Characterized all the three devices with the OD600 and fluorescence measurements in a Tecan micro-plate reader
- - Reported the results on the Interlab Study page of the iGEM wiki
- - Completed the description of the BBa_I20260 - Device 1
- - Submitted and send parts: BBa_K1403000 - Device 2 and BBa_K1403001 - Device 3 to the Biobricks registry
Collaborations
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut imperdiet diam eget quam imperdiet imperdiet. Mauris dapibus risus felis, sed ornare diam accumsan aliquet. Sed eu turpis porta, porttitor tortor et, condimentum augue. Curabitur a maximus nisi. Vivamus vitae magna ex. Donec congue auctor odio vitae tempus. In a gravida neque, et tristique tortor. Phasellus a odio sit amet enim ornare lobortis. Morbi sodales, diam non rutrum aliquam, ligula mauris consectetur urna, sed interdum quam risus sit amet enim. Aenean euismod enim magna, id pretium eros molestie non. Proin rutrum lobortis leo, sit amet congue erat. Nulla congue pellentesque augue porta dignissim. Pellentesque quis ex sollicitudin, condimentum risus varius, aliquet ipsum. Ut pulvinar aliquet maximus. Praesent imperdiet interdum commodo.
+33 1 44 41 25 22/25
paris-bettencourt-igem@googlegroups.com 
