Team:Groningen/Template/MODULE/Notebook/secretion/Introduction

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When we discovered that we did not have the desired Biobricks in the registry, we decided to design and characterize our own Biobricks. We wanted to characterize the superfolded GFP which was optimized for <i>Lactococcus lactis</i> and the CP promoter collection. We made the constructs containing the CP promoter collection with the super folded GFP. Although the superfolded GFP was optimized for <i>L. lactis</i>, it works in <i>E.coli</i> as well. The promoter collection was studied in <i>E.coli</i> by measuring the fluorescence in FACS. Based on the FACS data we analyzed the promoter activity of the CP promoter collection and proved superfolded GFP works well in <i>E.coli</i> too. One of our new Biobricks, NisA, was characterized by putting it before a CP44 promoter. NisA was then transformed to <i>Lactococcus lactis</i> and a nisin activity test was done. But unfortunately we could not see any zone of inhibition.
When we discovered that we did not have the desired Biobricks in the registry, we decided to design and characterize our own Biobricks. We wanted to characterize the superfolded GFP which was optimized for <i>Lactococcus lactis</i> and the CP promoter collection. We made the constructs containing the CP promoter collection with the super folded GFP. Although the superfolded GFP was optimized for <i>L. lactis</i>, it works in <i>E.coli</i> as well. The promoter collection was studied in <i>E.coli</i> by measuring the fluorescence in FACS. Based on the FACS data we analyzed the promoter activity of the CP promoter collection and proved superfolded GFP works well in <i>E.coli</i> too. One of our new Biobricks, NisA, was characterized by putting it before a CP44 promoter. NisA was then transformed to <i>Lactococcus lactis</i> and a nisin activity test was done. But unfortunately we could not see any zone of inhibition.

Revision as of 02:23, 18 October 2014

 
 
 

When we discovered that we did not have the desired Biobricks in the registry, we decided to design and characterize our own Biobricks. We wanted to characterize the superfolded GFP which was optimized for Lactococcus lactis and the CP promoter collection. We made the constructs containing the CP promoter collection with the super folded GFP. Although the superfolded GFP was optimized for L. lactis, it works in E.coli as well. The promoter collection was studied in E.coli by measuring the fluorescence in FACS. Based on the FACS data we analyzed the promoter activity of the CP promoter collection and proved superfolded GFP works well in E.coli too. One of our new Biobricks, NisA, was characterized by putting it before a CP44 promoter. NisA was then transformed to Lactococcus lactis and a nisin activity test was done. But unfortunately we could not see any zone of inhibition.


 
 
 
 

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