Team:UFMG Brazil/Project/Notebook

From 2014.igem.org

(Difference between revisions)

Revision as of 02:16, 18 October 2014

February
1st week	*First meeting with the team
2nd week
3rd week
4th week
5th week
March
1st week
2nd week
3rd week	Possibilities of themes: → Metabolic syndrome, → Promoter sensitive to sound → Use of yeast Doodle Description Trying to get support from Pro-reitoria→ Professor. Adelina *Sponsorship
4th week	*Defined theme: →Use of yeast for detection and treatment of colon cancer
5th week	*Vakinha (Crowd funding)
April
1st week	*Defined theme: →Use of yeast for detection and treatment of colon cancer
2nd week	Cancer of colon Probiotic: UFMG 905 Biomarkers: sensors Human practices (Meagan) → Difficulties to do Science in Brazil →Documentary → Buttons "I'm from Latin America" → Lectures
3rd week	*Identification system Colon cancer (Carlos) →Quantify the DNA present in the feces of mice with cancer (Laila) →Kit for DNA extraction from faeces (Rodrigo); →Disclosure TV radio and medias →Refresh the iGEM Facebook page →Search for new options field of non-specific DNA binding
4th week	Long strand DNA →Extracting DNA from faeces of mice →System Chimera proteins: look at other teams * Importance of attendance at team meetings * Romario: looking press office Oncologists →Situation of colon cancer →Diagnosis CCSP-2 (Oncogenes 2005) → Von Willebrand →Search for markers →DNA (Carlos) →Carbohydrates (Laila / Isadora / Lucas) →CCSP-2 (Rodrigo and Michele) →Metabolites (Marcelo and Dani) Need to find for sponsorship (Ciça and Chris) Idea to elaborate questionnaire to other Latin American iGEM teams (Thiago and Veronica)
5th week	TALE: Specific Sequence. DNA binding mCherry split: NEW !!! Can we use FRET techniques? mCherry modeling Microsatellite Synthesis of template DNA repeat Ca-binding proteins overexpressed ++ Biosafety CCSP-2: two domains von wilebrand Workers yeasts: central information (more than one signal "warning" the same cell)
May
1st week	*Division of the responsibilities: →Draw the biobricks (Carlos) →Collecting mice faeces (Laila) →Protocol for DNA extraction from faeces and quantification (Rodrigo and Michele) →Human Practices (Thiago Mafra) →Cancer in the past IGEM (Marcelo and Dani Chame) →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) →Sponsors (Cecilia, Chris, James and Veronica Bruno) →Translate questionnaire to Spanish (Dani Chame)
2nd week	Conversation with Paulo Sérgio Lacerda Beirão →Legislation of the country Videos with testimonials about the science in the country of researchers
3rd week	Metabolic Syndrome - network of diseases Biomarkers choice → limit →Complexity →Physiology →Stability Lenght DNA→ eletricity (3’ 5’) Accumulation of signal Transfer of fluorescence DNA bound proteins: proteins crawling in the DNA Noise/signal ratio: important for qualitative diagnosis Stool: noise Proximity can allow interaction with domains that will activate an enzyme that transform a smelly (banana?) substract OR Smell constitutive? Yeast die if it dos not meet signal. Difference between short and long DNA NEW idea: sound detection. mCherry
4th week	Protocol for feces Modeling (Gastone) →Probability of false positives Yeast plasmids design Paratope Fab / Histone *Validation in E.coli: pass after apra yeast synthesize: →TALE →mCherry →Long repetitive DNA →Short repetitive DNA
5th week
June
1st week	Workshop Biosynth How to earn money? →Brazilian companies? →INCT →Proreitoria →Mario Penna *Latin America Fan Page
2nd week	RFC10 - Biobrick standard Bsmb1 → TALE Software Assembly? Wiki Wet lab - gene synthesis How to earn money?
3rd week	Discount in the synthesis Enzymes: XbaI, SpeI, EcoR1, Pst1, AgeI, NgomIV, BsmbI →Phoneutria? Jacques Nicoli→ Yeast Biosafety (term 23/06) *Donation Fiocruz
4th week	New England Enzyme Texts Wiki →Project →Team →Human practices →Facebook →Vakinha (Crowd funding)
5th week
July
1st week	* Experimental tests * shopping * Restriction enzymes * length of 110 nucleotides linker → → try a smaller experimental →Biobrick assemble the plasmid →Sequencing →Express and perform the tests in vitro. →Write protocols for wiki Modeling mathematics Wiki: →Assemble layout and content. Human Practices →Questionnaires →Sponsorships →Questions - students ufmg schools. *Bioethics
2nd week	Price for synthesizing biobrick →Request assistance pro-reitoria Modeling →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 *Making a letter for sponsorship
3rd week	Deadlines Fundraising Ideas * Human Practices → Send e-mails with the questionnaires * Modeling of proteins *Extraction of faeces
4th week
5th week
August
1st week	* Arrival of restriction enzyme → Work Texts for wiki inscription IGEM
2nd week	*Experiments
3rd week	*Sponsors
4th week	Experiments Human Practices →Results of the questionnaires * Modeling of proteins
5th week
September
1st week	Meeting with Dr.Clélio Campolina, National minister of Science and Technology Wiki
2nd week	Human Practices: low adhesion Fetch data from investment in research Participation in Latin America over the years in the iGem Plot: X number of contacts feedback Lack of appreciation of Science Popular search
3rd week
4th week	Experiments Project Summary *Meeting with Dr. Evaldo Vilela, FAPEMIG director.
5th week
October
1st week	Finish the Wiki Modeling Experimental *Human practices. →Questionnaire in the 7 square
2nd week	Lecture to high school, Technical school and Graduation, at CEFET. Survey: The Importance of Science * Concentrations DNA * Sponsors * Deadlines
3rd week	* Experiments →Results * Human Practices →Results Modeling of proteins →Results Modeling mathematics →Results * Sponsors →Tshirt →Who helped?
4th week
5th week
November
	World Championship Jamboree.

@@ Line 18: / Line 18: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*First meeting with the team</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e"> </td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph"> </td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e"> </td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 42: / Line 42: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph"> </td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e"> </td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Possibilities of themes: <br>    → Metabolic syndrome, <br>    → Promoter sensitive to sound <br>    → Use of yeast <br>    *Doodle <br>    *Description <br>    *Trying to get support from Pro-reitoria→ Professor. Adelina <br>    *Sponsorship</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph">*Vakinha (Crowd funding)</td>
    </tr>
@@ Line 66: / Line 66: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*Defined theme:<br>    →Use of yeast for detection and treatment of colon cancer</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Cancer of colon<br>    *Probiotic: UFMG 905 <br>    *Biomarkers: sensors <br>    *Human practices (Meagan)<br>    → Difficulties to do Science in Brazil<br>    →Documentary <br>    → Buttons "I'm from Latin America" <br>    → Lectures</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Identification system Colon cancer (Carlos) <br>    →Quantify the DNA present in the feces of mice with cancer (Laila) <br>    →Kit for DNA extraction from faeces (Rodrigo); <br>    →Disclosure TV radio and medias <br>    →Refresh the iGEM Facebook page <br>    →Search for new options field of non-specific DNA binding</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*Long strand DNA <br>     →Extracting DNA from faeces of mice<br>  <br>     →System <br>     *Chimera proteins: look at other<br>  teams <br>     * Importance of attendance at team<br>  meetings <br>     * Romario: looking press office<br>  <br>     *Oncologists <br>     →Situation of colon cancer <br>     →Diagnosis <br>     *CCSP-2 (Oncogenes 2005) → Von<br>  Willebrand <br>     →Search for markers <br>     →DNA (Carlos) <br>     →Carbohydrates (Laila / Isadora /<br>  Lucas) <br>     →CCSP-2 (Rodrigo and Michele) <br>     →Metabolites (Marcelo and Dani)<br>  <br>     *Need to find for sponsorship (Ciça<br>  and Chris) <br>     *Idea to elaborate questionnaire to<br>  other Latin American iGEM teams (Thiago and Veronica)</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph">*TALE: Specific Sequence. DNA binding <br>     *mCherry split: NEW !!! <br>     *Can we use FRET techniques? <br>     *mCherry modeling <br>     *Microsatellite <br>     *Synthesis of template DNA repeat<br>  <br>     *Ca-binding proteins overexpressed<br>  ++ <br>     *Biosafety <br>     *CCSP-2: two domains von wilebrand<br>  <br>     *Workers yeasts: central information<br>  (more than one signal <br>    "warning" the same cell)</td>
    </tr>
@@ Line 90: / Line 90: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*Division of the responsibilities:<br>    →Draw the biobricks (Carlos) <br>    →Collecting mice faeces (Laila) <br>    →Protocol for DNA extraction from faeces and quantification (Rodrigo and<br>  Michele) <br>    →Human Practices (Thiago Mafra) <br>    →Cancer in the past IGEM (Marcelo and Dani Chame) <br>    →Video animation of IGEM UFMG BRAZIL (Lucas and Isadora) <br>    →Sponsors (Cecilia, Chris, James and Veronica Bruno) <br>    →Translate questionnaire to Spanish (Dani Chame)</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Conversation with Paulo Sérgio Lacerda Beirão<br>    →Legislation of the country<br>    *Videos with testimonials about the science in the country of researchers</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Metabolic Syndrome - network of diseases<br>     *Biomarkers choice → limit<br>     →Complexity<br>     →Physiology<br>     →Stability<br>     *Lenght DNA→ eletricity (3’<br>  5’)<br>     *Accumulation of signal<br>     *Transfer of fluorescence<br>     *DNA bound proteins: proteins<br>  crawling in the DNA<br>     *Noise/signal ratio: important for<br>  qualitative diagnosis<br>     *Stool: noise<br>     *Proximity can allow interaction<br>  with domains that will activate an enzyme that transform a smelly (banana?)<br>  substract<br>     OR<br>     *Smell constitutive?<br>     *Yeast die if it dos not meet<br>  signal.<br>     *Difference between short and long<br>  DNA<br>     *NEW idea: sound detection.<br>     *mCherry</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*Protocol for feces<br>    *Modeling (Gastone)<br>    →Probability of false positives <br>    *Yeast plasmids design <br>    *Paratope Fab / Histone <br>    *Validation in E.coli: pass after apra yeast synthesize:<br>    →TALE <br>    →mCherry <br>    →Long repetitive DNA <br>    →Short repetitive DNA</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 114: / Line 114: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*Workshop Biosynth <br>     *How to earn money? <br>     →Brazilian companies? <br>     →INCT <br>     →Proreitoria <br>     →Mario Penna <br>     <br>     *Latin America Fan Page</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*RFC10 - Biobrick standard <br>     *Bsmb1 → TALE <br>     *Software Assembly? <br>     *Wiki <br>     *Wet lab - gene synthesis <br>     *How to earn money?</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Discount in the synthesis <br>     *Enzymes: XbaI, SpeI, EcoR1, Pst1,<br>  AgeI, NgomIV, BsmbI <br>     →Phoneutria? <br>     *Jacques Nicoli→ Yeast <br>     *Biosafety (term 23/06) <br>     *Donation Fiocruz</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*New England Enzyme <br>     *Texts Wiki <br>     →Project <br>     →Team <br>     →Human practices <br>     →Facebook<br>     →Vakinha (Crowd funding)</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 138: / Line 138: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">* Experimental tests <br>    * shopping<br>    * Restriction enzymes <br>    * length of 110 nucleotides linker → → try a smaller <br>    *experimental <br>    →Biobrick assemble the plasmid <br>    →Sequencing<br>    →Express and perform the tests in vitro. <br>    →Write protocols for wiki <br>    *Modeling mathematics <br>    *Wiki: <br>     →Assemble layout and content. <br>    *Human Practices <br>    →Questionnaires <br>    →Sponsorships <br>    →Questions - students ufmg schools. <br>    *Bioethics</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Price for synthesizing biobrick <br>    →Request assistance pro-reitoria <br>    <br>    *Modeling <br>    →test of interaction assemblies TALE + + Linker Hmcherry1 and 2 <br>    *Making a letter for sponsorship</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Deadlines<br>    * Fundraising Ideas <br>    * Human Practices <br>    → Send e-mails with the questionnaires <br>    * Modeling of proteins <br>    *Extraction of faeces</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e"> </td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 162: / Line 162: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">* Arrival of restriction enzyme <br>    → Work<br>    *Texts for wiki <br>    *inscription IGEM</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Experiments</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">*Sponsors</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*Experiments<br>    * Human Practices <br>    →Results of the questionnaires<br>    * Modeling of proteins</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 186: / Line 186: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*Meeting with Dr.Clélio Campolina, National minister of<br>  Science and Technology<br>    *Wiki</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Human Practices: low adhesion <br>    *Fetch data from investment in research <br>    *Participation in Latin America over the years in the iGem<br>    *Plot: X number of contacts feedback <br>    *Lack of appreciation of Science <br>    *Popular search</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph"> </td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e">*Experiments<br>    *Project Summary<br>     *Meeting with Dr. Evaldo Vilela,<br>  FAPEMIG director.</td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>
@@ Line 210: / Line 210: @@
    </tr>
    <tr>
-     <td class="tg-4eph">1º week</td>
+     <td class="tg-4eph">1st week</td>
      <td class="tg-4eph">*Finish the Wiki <br>    Modeling <br>    *Experimental <br>    *Human practices. <br>    →Questionnaire in the 7 square</td>
    </tr>
    <tr>
-     <td class="tg-031e">2º week</td>
+     <td class="tg-031e">2nd week</td>
      <td class="tg-031e">*Lecture to high school, Technical school and Graduation, at<br>  CEFET.<br>    * Survey: The Importance of Science <br>    * Concentrations DNA <br>    * Sponsors <br>    * Deadlines</td>
    </tr>
    <tr>
-     <td class="tg-4eph">3º week</td>
+     <td class="tg-4eph">3rd week</td>
      <td class="tg-4eph">* Experiments <br>    →Results <br>    * Human Practices <br>    →Results <br>    *Modeling of proteins<br>    →Results <br>    * Modeling mathematics <br>    →Results <br>    * Sponsors <br>    →Tshirt <br>    →Who helped?</td>
    </tr>
    <tr>
-     <td class="tg-031e">4º week</td>
+     <td class="tg-031e">4th week</td>
      <td class="tg-031e"> </td>
    </tr>
    <tr>
-     <td class="tg-4eph">5º week</td>
+     <td class="tg-4eph">5th week</td>
      <td class="tg-4eph"> </td>
    </tr>