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Team:Bordeaux/Results
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| - | [[file:Bdx2014 Production.jpg|left|400px]] [https://2014.igem.org/Team:Bordeaux/Results/Production Production (click for more info)]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | + | [[file:Bdx2014 Production.jpg|left|400px]] [https://2014.igem.org/Team:Bordeaux/Results/Production Production (click for more info)] We managed to settle the best conditions to produce the biopolymers in maximal amount. Different medium were tested, a special BL21 strai was used.<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> |
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[[File:Bdx2014 Purification.jpg|left|400px]] [https://2014.igem.org/Team:Bordeaux/Results/Purification Purification (click for more info)]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | [[File:Bdx2014 Purification.jpg|left|400px]] [https://2014.igem.org/Team:Bordeaux/Results/Purification Purification (click for more info)]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
Revision as of 02:15, 18 October 2014
References
[1] Doreen M. Floss et al. ELASTIN-like polypeptides revolutionize recombinant protein expression and their biomedical application. Trends in Biotechnology Vol.28 No.1 (PMID 19897265)
[2] Dan W. Urry Entropic Elastic Processes in Protein Mechanisms. I. Elastic Structure Due to an Inverse Temperature Transition and Elasticity Due to Internal Chain Dynamics. Journal of Protein Chemistry, Vol. 7, No.. I, 1988
[3] Dan W. Urry. Physical Chemistry of Biological Free Energy Transduction As Demonstrated by Elastic Protein-Based Polymers. J. Phys. Chem. B 1997, 101, 11007-11028
[4] Dan E. Meyer and Ashutosh Chilkoti. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. NATURE BIOTECHNOLOGY VOL 17 NOVEMBER 1999
[5] Trabbic-Carlson et al. (2004), Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein Science, 13: 3274–3284. doi: 10.1110/ps.04931604
[6] K. Trabbic‐Carlson et al. Effect of protein fusion on the transition temperature of an environmentally responsive elastin‐like polypeptide: a role for surface hydrophobicity? Protein Engineering, Design and Selection (2004) 17 (1): 57-66. doi: 10.1093/protein/gzh006
