Team:Evry/Notebook/Transformation/09-10-2014

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<u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br>
<u> <b> <big><FONT COLOR=#003333>Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation </font></big></b> </u> <br>
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A contamination has been shown in another transformation test. We can't use our previous results because there is a chance that it's not <i>Pseudovibrio</i> but the contamination which had been transformed.
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The previous digestion could fail because of the too little time of incubation (15min). We re-try to make the digestion with an incubation of 1 hour. <br>
 
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    <center>Gel of digestion with XbaI (2)</center>
 
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There was a problem with the digestion. We are going to try with another enzyme.<br>
 
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<u> <b> Stock of pBBR1MCS and pRhokHI-2 </b> </u> <br>
<u> <b> Stock of pBBR1MCS and pRhokHI-2 </b> </u> <br>
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The colony of E.Coli transformed in LB 1X + Cam (1:1000) culture of 3mL was sowed on new LB/LB+Cam(1:1000)/LB+Kan(1:1000) plates.<br>
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The colony of E.Coli transformed in LB 1X + Cam (1:1000) culture of 3mL was sowed on new LB/LB+Cam(1:1000)/LB+Kan(1:1000) plates and incubated with shaking overnight at 37°C.<br>
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Revision as of 01:48, 18 October 2014

Picture

Transformation of Pseudovrbrio with pBBR1MCS and pRhokHI-2 - Confirmation
A contamination has been shown in another transformation test. We can't use our previous results because there is a chance that it's not Pseudovibrio but the contamination which had been transformed.
Stock of pBBR1MCS and pRhokHI-2
The colony of E.Coli transformed in LB 1X + Cam (1:1000) culture of 3mL was sowed on new LB/LB+Cam(1:1000)/LB+Kan(1:1000) plates and incubated with shaking overnight at 37°C.

Sep 10

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