Team:British Columbia/ProjectBiomining

From 2014.igem.org

(Difference between revisions)
Line 14: Line 14:
'''Our solution''':
'''Our solution''':
-
We, the UBC iGEM team, feel that separation and enrichment can be done in other ways that does not rely on energy or chemically-intensive methods. Our synbio solution involves the use of small surface heptapeptides and octapeptides, which have previously been demonstrated in M13 bacteriophage by Curtis et al. to selectively bind to chalcopyrite(3). Three peptides,TPTTYKV, DSQKTNPS, DPIKHTSG, have been identified for binding. Operating with bacteriophage, however, is not feasible for large scale operations as it is difficult to scale up titers to cover for the smaller surface area available for binding in bacteriophage. Our idea is to operate these peptides in bacteria which have a larger surface area and are much more responsive to stimuli. These peptides must be accessible on the surface. The chassis  The end goals of this project are to bind selectively to chalcopyrite from ore slurries containing chalcopyrite and impurities such as enargite.  
+
We, the UBC iGEM team, feel that separation and enrichment can be done in other ways that does not rely on energy or chemically-intensive methods. Our synbio solution involves the use of small surface heptapeptides and octapeptides, which have previously been demonstrated in M13 bacteriophage by Curtis et al. to selectively bind to chalcopyrite(3). Three peptides, labelled WSD-1 (TPTTYKV), WSD-2 (DSQKTNPS), and WSD-3 (DPIKHTSG), have been identified for binding. However, operating with bacteriophage is not feasible for large scale operations in mining as it is difficult to scale up titers to compensate for the smaller surface area available for binding in bacteriophage. Our idea is to operate these peptides in bacteria which have a larger surface area and are much more responsive to stimuli. These peptides must be accessible on the surface. Therefore, we have chosen ''Caulobacter crescentus'' as the chosen chassis given that it contains a S-protein layer in which we can express our peptides. A developed kit for protein secretion and display of peptides on the cell surface S-layer in ''Caulobacter crescentus''  (via cloning to the S-layer gene sequence) can be found at [http://ubc.flintbox.com/public/project/1487/ Caulobacter S-layer Kits]
 +
 
 +
A novel protein secretion and display system, using Caulobacter crescentus, that provides scalable, inexpensive secretion of recombinant proteins (via fusion with a C-terminal secretion signal) or display of peptides or proteins on the cell surface (via insertion into the complete S-layer gene sequence). Caulobacter expressing the immunoglobulin G-binding domain of Streptococcal Protein G is available for purchase.    
 +
 
 +
 
 +
 
 +
The end goals of this project are to bind selectively to chalcopyrite from ore slurries containing chalcopyrite and impurities such as enargite.  

Revision as of 01:39, 18 October 2014

2014 UBC iGEM

© 2014 UBC iGEM