Team:ITESM-CEM/Parts
From 2014.igem.org
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- | <p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to degrade 7-ketocholesterol consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related | + | <p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to degrade 7-ketocholesterol consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis. |
</p><br> | </p><br> | ||
<h2>Cholesterol Oxidase</h2> | <h2>Cholesterol Oxidase</h2> | ||
- | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in Chromobacterium sp. We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance. Its length is of 1871 nucleotides and its codons were optimized in order to use it on E.coli, it already included a stop codon, it was also modified by the addition of a glycosilation site and the peptide signal of human cathepsin. |
</p> <br> | </p> <br> | ||
Revision as of 01:45, 18 October 2014
ITESM-CEM | Enzy7-K me |
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