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Team:Paris Saclay/Notebook/July/17
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===3 - PCR and Electrophoresis=== | ===3 - PCR and Electrophoresis=== | ||
Revision as of 15:43, 21 July 2014
Contents |
Thursday 17th July
Lab work
1 - Preparation of supercompetent cells
By Mathias & Romain
Strains used: MG1655 (x2), MG1655Z1 (x2).
2 - Bacterial Culture
by Fabio and Maher
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both J23119 from the concentrated colony made the 16th July
3 - PCR and Electrophoresis
by Arnaud & Mathias
PCR targeting with oligos IPS70 and IPS71.
- Molecular weight marker 1kb
- Empty
- IPS70 and IPS71 (1)
- IPS70 and IPS71 (2)
People there:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
