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From 2014.igem.org

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Revision as of 00:25, 18 October 2014

p14rz_002

• PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
• Digest: pKM006, EcoRI + NotI; PCR-product
• Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7

p14rz_003

• PCR 3
• Digest 3, 4
• Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8

p14rz_004

• PCR 21,22
• Digest -
• Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2

p14rz_005

• PCR 4,5
• Digest 5
• Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3

p14rz_006

• PCR 17,18
• Digest 5
• Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4

p14rz_007

• PCR 37,38
• Digest 21
• Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7

p14rz_008

• PCR blue light receptor; Template: pQXCIN-SLC7A1
• Digest pKM006 EcoRI, NotI
• Testdigest with SmaI in Cutsmart, expected bands: 5,7

p14rz_009

• PCR 39,40
• Digest 5
• Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7

p14rz_010

• PCR 23,24
• Digest 12
• Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56

p14ls_003

• PCR 7,9,10
• Digest -
• Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4

p14vv_001

• PCR Primers vv003,vv004; Template: pMIG
• Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
• Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6

p14vv_002

• PCR -
• Digest 8,11
• Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4

p14vv_003

• PCR pMIG with primer vv003+vv004-
• Digest xx
• Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5

p14vv_006

• PCR 26
• Digest 13
• Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6

p14vv_007

• PCR 27
• Digest 14
• Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66

p14vv_008

• PCR 43
• Digest 22
• Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75

p14vv_009

• PCR 44
• Digest 22
• Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75

p14vv_010

• PCR 45
• Digest 22
• Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2

p14vv_012

• PCR 46
• Digest 22
• Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4

p14vv_013

• PCR 47,48,49
• Digest 22
• Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2

p14vv_014

• PCR 50,51
• Digest 22
• Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66

p14zl_010

• PCR 1,2
• Digest 1
• Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1

p14zl_011

• PCR 19,20
• Digest 1
• Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43

p14zl_016

• PCR 52,53
• Digest 22
• Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7

p14zl_017

• PCR 64,65
• Digest 24
• testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9

p14zl_020

• PCR 59,60
• Digest 24
• testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8