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Team:British Columbia/Notebook/Protocol/Biobrickdigest
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*depending on DNA concentration* | *depending on DNA concentration* | ||
Revision as of 23:28, 17 October 2014
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BioBrick Restriction Digest
Supplies:
- dsH2O
- 10x buffer (1,2,3,4)
- 100x BSA
- Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)
- DNA
Steps:
- Make a master mix with everything except the DNA. Keep everything on ice.
- Pipet aliquots to each sample tube and add DNA.
- Incubate at 37°C for 2-3h or let digest over night at room temperature.
*depending on DNA concentration*
