Team:DTU-Denmark/Achievements/Calculator

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PoPS calculator V. 1.0
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This calculator allows you to calculate your promoter activity in PoPS (Polymerases Per Second)
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In order to use it you need to obtain:
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Fluorescence signal from your culture.
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Fluorescence per spinach (slope of a standard series).
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Background fluorescence from your culture (intercept).
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CFU/L or optical density at 600 nm (OD600) for your measured culture.
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We have found degradation rate of Spinach in <i>E. coli</i> when grown in LB at 37 &deg;C  so feel free to use this value.
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You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell.
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When your values are entered to the calculator you will receive your result, the calculator works using the formula derived <a href”https://2014.igem.org/Team:DTU-Denmark/Achievements/Modelling”>here</a>
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Revision as of 23:34, 17 October 2014

Technical University of Denmark

Fluorescence:
Standard curve, intercept:
Standard curve, slope:
Spinach concentration: µM
Input concentration directly
PoPS calculator V. 1.0 This calculator allows you to calculate your promoter activity in PoPS (Polymerases Per Second) In order to use it you need to obtain: Fluorescence signal from your culture. Fluorescence per spinach (slope of a standard series). Background fluorescence from your culture (intercept). CFU/L or optical density at 600 nm (OD600) for your measured culture. We have found degradation rate of Spinach in E. coli when grown in LB at 37 °C so feel free to use this value. You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell. When your values are entered to the calculator you will receive your result, the calculator works using the formula derived here
CFU count CFU/L
Use OD measurement
OD600
Degradation rate:
Use degradation half time
Degradation half time:
Growth rate:
Use growth doubling time
Growth doubling time:
Fold-fraction: %
Copy number:
Promoter activity PoPS








Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .

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