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Team:Northwestern/CFPS
From 2014.igem.org
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<li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | <li>out of K12, DH5a, and BL21, BL21 expressed the most fluorescence</li> | ||
<li>the plasmid used was a construct of superfolder GFP from the Jewett Lab</li> | <li>the plasmid used was a construct of superfolder GFP from the Jewett Lab</li> | ||
| - | <li><b>conclusion</b>our lysates are functional</li> | + | <li><b>conclusion:</b>our lysates are functional</li> |
</ul> | </ul> | ||
Revision as of 22:45, 17 October 2014
Cell-Free Protein Synthesis
20140723 Testing our lysates
- data from BL21 (E.Coli) lysates
- out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
- the plasmid used was a construct of superfolder GFP from the Jewett Lab
- conclusion:our lysates are functional
20140915 All of our lysates
- RBS B0034
- Tried all of our lysates
- green fluorescence indicates mRNA levels of transcription
- red fluorescence indicates protein levels of translation
- conclusion: the data is most likely statistically insignificant
20141014 Cell-Free Protein Synthesis Assay with All of our Lysates
- We used RBS No. 5 (B0034) with RFP-Spinach Aptamer
- Displayed here is the first read
- The kinetic portion of the plate reader did not record data in all of our wells
- We will continue doing CFPS experiments
- conclusion: we need more time! Looks like fluorophores were incorrectly added
