Team:Oxford/modellingA

From 2014.igem.org

(Difference between revisions)
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     table.t1 {border-collapse: collapse}
     table.t1 {border-collapse: collapse}
     td.td1 {width: 63.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td1 {width: 63.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
-
     td.td2 {width: 66.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td2 {width: 69.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td3 {width: 93.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td3 {width: 93.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td4 {width: 81.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td5 {width: 59.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td5 {width: 59.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
-
     td.td6 {width: 80.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td6 {width: 58.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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+
     td.td7 {width: 80.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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+
     td.td8 {width: 50.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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+
     td.td9 {width: 43.0px; height: 23.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td10 {width: 63.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td11 {width: 66.0px; height: 12.0px; background-color: #9ce15a; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td11 {width: 69.0px; height: 12.0px; background-color: #9ce15a; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td12 {width: 93.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td12 {width: 93.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td13 {width: 81.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
     td.td13 {width: 81.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td14 {width: 59.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td15 {width: 80.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td15 {width: 58.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
-
     td.td16 {width: 69.0px; height: 12.0px; background-color: #64b2df; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td16 {width: 80.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td17 {width: 49.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
    td.td17 {width: 69.0px; height: 12.0px; background-color: #64b2df; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td18 {width: 42.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td18 {width: 50.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td19 {width: 63.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td19 {width: 43.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td20 {width: 66.0px; height: 11.0px; background-color: #9ce15a; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td20 {width: 63.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td21 {width: 93.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td21 {width: 69.0px; height: 11.0px; background-color: #9ce15a; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td22 {width: 81.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td22 {width: 93.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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+
     td.td23 {width: 81.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td24 {width: 80.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td24 {width: 59.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td25 {width: 69.0px; height: 11.0px; background-color: #64b2df; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
+
     td.td25 {width: 58.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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+
     td.td26 {width: 80.0px; height: 11.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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     td.td27 {width: 69.0px; height: 11.0px; background-color: #64b2df; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
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 +
     td.td30 {width: 69.0px; height: 12.0px; border-style: solid; border-width: 1.0px 1.0px 1.0px 1.0px; border-color: #000000 #000000 #000000 #000000; padding: 4.0px 4.0px 4.0px 4.0px}
   </style>
   </style>
</head>
</head>

Revision as of 08:10, 17 July 2014

[DCM] calibration for solution-vapour equilibration

Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:




V(DCM) added (± 5E-4) /ml

n(DCM)(l) unadjusted (± 8E-6) /mol

[DCM] unadjusted (± 2E-3) /M

p(DCM) 

(± 4E-2) /kPa

n(DCM)(g)

(± 4E-7) /mol

n(DCM)(l) (± 9E-6) adjusted /mol

[DCM] adjusted 

(± 2E-3) /M

Δ[DCM] /M

[DCM] % deviation

(30°C, 5mM)

1.6E-03

2.5E-05

5.0E-03

6.2E-01

1.6E-08

2.5E-05

5.0E-03

3.1E-06

6.3E-02

(30°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.9E-09

5.0E-05

1.0E-02

1.6E-06

1.6E-02

(30°C, 20mM)

6.4E-03

1.0E-04

2.0E-02

1.6E-01

3.9E-09

1.0E-04

2.0E-02

7.9E-07

3.9E-03











(37°C, 5mM)

1.6E-03

2.5E-05

5.0E-03

6.2E-01

1.5E-08

2.5E-05

5.0E-03

3.1E-06

6.2E-02

(37°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.7E-09

5.0E-05

1.0E-02

1.5E-06

1.5E-02

(37°C, 20mM)

6.4E-03

1.0E-04

2.0E-02

1.6E-01

3.8E-09

1.0E-04

2.0E-02

7.7E-07

3.9E-03


More detailed system modelling is therefore unnecessary.