Team:Evry/Notebook/Transformation/08-28-2014
From 2014.igem.org
(Difference between revisions)
Line 10: | Line 10: | ||
- | <u | + | <u> <big><FONT COLOR=#003333><b> Amplification of pRhokHI-2 in E.Coli Bl21 - Results</b> </font></big> </u> <br> |
After incubation overnight we can see some colonies of E.Coli Bl21 transformed on LB+Cam 1:1000 and on LB+Kan 1:1000. Our Bl21 which had not been transformed doesn't growth on these media but both grow on LB only. | After incubation overnight we can see some colonies of E.Coli Bl21 transformed on LB+Cam 1:1000 and on LB+Kan 1:1000. Our Bl21 which had not been transformed doesn't growth on these media but both grow on LB only. | ||
We had well success to tranform E.Coli and amplifly the plasmid. | We had well success to tranform E.Coli and amplifly the plasmid. |
Latest revision as of 22:22, 17 October 2014
![Picture](https://static.igem.org/mediawiki/2013/9/90/EvryBiology.png)
Amplification of pRhokHI-2 in E.Coli Bl21 - Results
After incubation overnight we can see some colonies of E.Coli Bl21 transformed on LB+Cam 1:1000 and on LB+Kan 1:1000. Our Bl21 which had not been transformed doesn't growth on these media but both grow on LB only.
We had well success to tranform E.Coli and amplifly the plasmid.
![Transfo2](https://static.igem.org/mediawiki/2014/0/09/Pouet.jpg)
We tranfered two colonies into liquid MB+Kan(1:1000) and let them in incubator overnight to have a pre-culture for the plasmid purification with NucleoSpin Plamsid Kit (Macherey Nagel).
Aug 28