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Revision as of 22:06, 17 October 2014
Attributions
We would like to thank all the people who came to our progress presentations and gave useful feedback.
Instructors support
Lin,Guang-Huey Professor Lin teaches knowledge about microbiology for TCU iGEM team each year, and she also guides our experimental design. Besides, she provides her lab for us to working on our experiment.
Lin,Nien-Tsung Prof. Lin, a gracious teacher, is the guiders of our experiment on M13 phage display. She provides us a lot knowledge about M13 phage and DNA cloning.
Ching,Yung-Hao Prof. Ching is one of the leader teachers of our team.
She is a kind teacher who always encourages us, and she also gives us much advice and direction on safety concerns.
Woon,Peng-Yeong Prof. Woon is one of the leader teachers for this year. He helps us the basic concept of iGEM and reminds us many iGEM rules or requests we nearly missed. He is a lively professor.
Experiment
Bobo, Regina, Denise, Eating
Contributions
Transformation ( Cas9 in pSB1C3 into DH5α) Extract plasmid with pSB1C3 from DH5α. Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011. Use Enzyme digestion (Pst I and EcoR I) to confirm whether pSB1C3 and pBluescript is successfully extracted. Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate. Ligation of RFP and pBluescript SK(-). Transformation clone RFP/pBluescript SK(-) to DH5α. Extract plasmid clone RFP/pBluescript SK(-).
Experiment Design Ted and Bobo
Design gRNA for AmpR.
Biobricks Design Ted
Design the gRNA for different resistance gene.
Modeling
House, Bobo, Chao-Di Chang(National Chiao Tung University)
Contributions
Infectional efficiency ratio of M13KO7. Release titer.(Releasing titer of phagemid which is E.coli infected by helper phage)
Special Thanks Modeling Advisor
Chang,Chao-Di
Thanks for Chang,Chao-Di strong support on our modeling.
Human Practice
Eating
Contributions
Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener. Contact with National Chiao Tung University for cooperation.
Wiki
House
Contributions
Construct the wiki page. Coding,coding and coding the wiki page. Study all about Synthetic Biology from wetlab to drylab.
And last but not least
Art Design Denise, Regina, Eating, House Design of poster and PPT.
Photo & Video Sharon, Eating Take photo and video.
Administeration Eating Apply for the allowances.
Video support
Wang, Yiru and Chen, Jiawei
we should be obliged to them for their assistance in this matter.
Wang, Yi-ru
Chen, Jia-wei
Special Thanks
Our partner : NCTU_Formosa
We are extremely appreciative of NCTU_Formosa that helped and support us. The model1 courtesy of Chao-Di Chang.
Sponsors
We have taken efforts in this project. However, it would not have been possible without the kind support and help of many individuals and organizations. We would like to extend our sincere thanks to all of them.
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