Team:Northwestern/CFPS

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Revision as of 22:04, 17 October 2014

Dropdown menu from bootstrap

Cell-Free Protein Synthesis

20140723 Testing our lysates
  • data from BL21 (E.Coli) lysates
  • out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
  • the plasmid used was superfolder GFP from Jewett Lab
  • conclusionour lysates are function
red: control, blue: BL21 lysate
20140915 All of our lysates
  • RBS B0034
  • Tried all of our lysates
  • green fluorescence indicates amount of transcription
  • red fluorescence indicates amount of translation
  • conclusion: the data is most likely statistically meaningless
20141014 Last CFPS attempt with all of our lysates
  • We used RBS no. 5 (B0034) with Spinach Aptamer
  • Displayed here is the first read
  • The kinetic portion of the plate reader did not record data in all of our wells
  • We will continue doing CFPS experiments
  • conclusion: we need more time! Looks like fluorophores were incorrectly added