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Team:Oxford/modellingA
From 2014.igem.org
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<h1>[DCM] calibration for solution-vapour equilibration</h1> | <h1>[DCM] calibration for solution-vapour equilibration</h1> | ||
<p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:</p> | <p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:</p> | ||
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| + | <math>k(T)=k^\Theta&space;e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" target="_blank"><img src="http://latex.codecogs.com/gif.latex?k(T)=k^\Theta&space;e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" title="k(T)=k^\Theta e^{\frac{\mathrm{d} lnk}{\mathrm{d} 1/T}(\frac{1}{T}-\frac{1}{298.15K})}" /></math> | ||
<p class="p1"><br></p> | <p class="p1"><br></p> | ||
Revision as of 17:40, 16 July 2014
[DCM] calibration for solution-vapour equilibration
Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a binary solution/ideal gas, based on literature solubility data, confirming negligible [DCM] deviation:
|
|
p(DCM) /kPa |
n(g) /mol |
V(DCM) added /ml |
n(DCM)(l) unadjusted /mol |
[DCM] unadjusted /M |
n(DCM)(l) adjusted /mol |
[DCM] adjusted /M |
[DCM] % deviation |
|
(30°C, 5mM) |
1.56E-04 |
3.94E-12 |
6.4E-03 |
1.0E-04 |
2.0E-02 |
1.0E-04 |
2.0E-02 |
3.9E-06 |
|
(30°C, 10mM) |
3.12E-04 |
7.88E-12 |
3.2E-03 |
5.0E-05 |
1.0E-02 |
5.0E-05 |
1.0E-02 |
1.6E-05 |
|
(30°C, 20mM) |
6.24E-04 |
1.58E-11 |
1.6E-03 |
2.5E-05 |
5.0E-03 |
2.5E-05 |
5.0E-03 |
6.3E-05 |
|
|
|
|
|
|
|
|
|
|
|
(37°C, 5mM) |
1.56E-04 |
3.94E-12 |
6.4E-03 |
1.0E-04 |
2.0E-02 |
1.0E-04 |
2.0E-02 |
3.9E-06 |
|
(37°C, 10mM) |
3.12E-04 |
7.88E-12 |
3.2E-03 |
5.0E-05 |
1.0E-02 |
5.0E-05 |
1.0E-02 |
1.6E-05 |
|
(37°C, 20mM) |
6.24E-04 |
1.58E-11 |
1.6E-03 |
2.5E-05 |
5.0E-03 |
2.5E-05 |
5.0E-03 |
6.3E-05 |
