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| <h4 style="color:#00A0E9;background-color:#eeeeee">Collaboration</h4></p> | | <h4 style="color:#00A0E9;background-color:#eeeeee">Collaboration</h4></p> |
- | <p><b>-National Yang-Ming University</b></p>
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- | <a href="https://2014.igem.org/Team:NYMU-Taipei"><img src="images/Ymedulogo.jpg" width="200px"></a></p>
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- | <h5 style="line-height:1.4em;font-size:110%;"><strong>We</strong> help registered iGEM team-National Yang-Ming University improve their project by debugging their construct, modeling their system through system we composed and testing result. Several meeting were held to communicate in both directions.</h5>
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- | <h4 style="color:gray">
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- | Function of signal protein YebF</h4>
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- | <h5 style="line-height:1.4em;font-size:110%;">
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- | <strong style="font-size:110%">Purpose:</strong></p>
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- | Testing if the signal sequence before the coding product can successfully work, bring the protein outside of the cell.</p>
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- | <strong>[Notice]</strong>Before reading further details, please notice that we have named these circuits with three symbols:</p>
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- | Comp=competent cell</p>
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- | J23100=J23100+B0034+RFP+B0015</p>
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- | J+Y+R+B=J23100+RBS-yebF+RFP+B0015</p>
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- | <strong style="font-size:110%">Method:</strong></p>
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- | <p style="padding-bottom:10px">1. Three E.coli strains is cultured overnight in 15mL LB medium respectively in 15ml centrifuge tube and incubate in 37C overnight. </p>
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- | 2. Adjusting all of the culture to an OD value of 0.6, and add 1ml of each into flasks containing 80ml LB.</p>
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- | <p style="padding-bottom:30px">3.Put all of them into 37C incubator and start the measurement.</p>
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- | Measurement:
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- | </p>
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- | <p style="padding-bottom:30px">
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- | For each sample : LB, competent cell, J23100, and J+Y+R+B,</p>
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- | And do quadruplication for each of them every certain time interval.
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- | </p>
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- | <strong style="font-size:110%">Result:</strong></p>
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- | <img src="images/yang%20ming%201.png" width="600px" style="padding-bottom:30px">
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- | <img src="images/yang%20ming%202.jpg" width="600px" style="padding-bottom:30px">
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- | <img src="images/yang%20ming%203.jpg" width="600px" style="padding-bottom:30px">
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| <h2 style="padding-bottom:100px"><a href="https://2014.igem.org/Team:NYMU-Taipei">WIKI Links</a></h2> | | <h2 style="padding-bottom:100px"><a href="https://2014.igem.org/Team:NYMU-Taipei">WIKI Links</a></h2> |
| </li> | | </li> |