Team:Oxford/Parts

From 2014.igem.org

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<u>Part no. 2 (BBa_K1446002)</u></a> is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected.
<u>Part no. 2 (BBa_K1446002)</u></a> is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected.
<img src="https://static.igem.org/mediawiki/parts/1/1d/BBa_k1446002.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" />
<img src="https://static.igem.org/mediawiki/parts/1/1d/BBa_k1446002.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" />
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<a href="http://parts.igem.org/Part:BBa_K1446003">
<a href="http://parts.igem.org/Part:BBa_K1446003">
<u>Part no. 3 (BBa_K1446003)</u></a> is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show2">
<u>Part no. 3 (BBa_K1446003)</u></a> is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described <a href="https://2014.igem.org/Team:Oxford/biosensor_characterisation#show2">
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<img src="https://static.igem.org/mediawiki/parts/8/80/BBa_k1446003.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" />
<img src="https://static.igem.org/mediawiki/parts/8/80/BBa_k1446003.jpg" style="float:left;position:relative; width:90%; margin-right:75%;margin-bottom:2%;" />
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Parts no. 1 and 2 belong to our bioremediation subproject and part 3 to our biosensor subproject.
Parts no. 1 and 2 belong to our bioremediation subproject and part 3 to our biosensor subproject.

Revision as of 21:25, 17 October 2014


Parts


Oxford iGEM has submitted three parts to the registry, which are described on the relevant registry pages.

Part no. 1 (BBa_K1446001) is the gene coding for DcmA with an N-terminal pdu-microcompartment tag.

Part no. 2 (BBa_K1446002) is a superfolder GFP-gene with the N-terminal pdu-microcompartment tag. We have included a ribosome binding site upstream of the coding region as well as a his-tag at the C-terminus. This part has been used for fluorescent image analysis to show that the targeting mechanism of the N-terminal microcompartment tag works as expected.

Part no. 3 (BBa_K1446003) is the dcmR gene with a tetR promoter. We have used this part to get fluorescence data for our model described here and showed that mCherry expression increased at higher ATC (anhydrous tetracycline) concentration, as expected.

Parts no. 1 and 2 belong to our bioremediation subproject and part 3 to our biosensor subproject.


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