Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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<p class="title4">Lab work:</p>
<p class="title4">Lab work:</p>
<p class="texte">
<p class="texte">
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- Chemotaxis test and modelling
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- Chemotaxis test and modeling
<br/>
<br/>
- Fungicides tests on EcAMP-A,  EcAMP-B, EcAMP-C, EcAMP-D, MaxiOpA, MaxiOpB, MaxiOpC, MaxiOpD from 2ml of overnight cultures. Only with 25000 conidia with both fungi.
- Fungicides tests on EcAMP-A,  EcAMP-B, EcAMP-C, EcAMP-D, MaxiOpA, MaxiOpB, MaxiOpC, MaxiOpD from 2ml of overnight cultures. Only with 25000 conidia with both fungi.
<br/>
<br/>
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- PCR on pSBBS4S + BBa_K1162001 (EcAMP)  
+
- PCR on pSB<sub>BS</sub>4S + BBa_K1162001 (EcAMP)  
<br/>
<br/>
Problem: failed on both diluted plasmid and colony
Problem: failed on both diluted plasmid and colony
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- PCR on D4E1 colony with different primers
- PCR on D4E1 colony with different primers
<br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion  
<br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion  
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<br/>- Transformation of K1364014+K1364006 in <i>E. coli</i>  
+
<br/>- Transformation of K1364014 + K1364006 in <i>E. coli</i>  
-
<br/>- Transformation of GAFP1 + D4E1, GAFP1 and  EcAMP+GAFP1+D4E1 on <i>B. subtilis</i> + colony PCR + threonine test
+
<br/>- Transformation of GAFP1 + D4E1, GAFP1 and  EcAMP + GAFP1 + D4E1 on <i>B. subtilis</i> + colony PCR + threonine test
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<br/>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1 CA
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<br/><i>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1</i>
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<br/>- Cloning of K606013+pEX_K4, 823022+PlepA_RFP, 823022+ Pveg_RFP
+
<br/>- Cloning of K606013 + pEX_K4, 823022 + P<sub>lepA</sub>_RFP, 823022 + P<sub>veg</sub>_RFP
<br/>- Chemotaxis tests: different times of LB + agar addition in the LB medium (after 3 minutes, 5 minutes, 8 minutes, 10 minutes…) are performed => No difference of the results after spreading on the plates.
<br/>- Chemotaxis tests: different times of LB + agar addition in the LB medium (after 3 minutes, 5 minutes, 8 minutes, 10 minutes…) are performed => No difference of the results after spreading on the plates.
<br/>- New test of chemotaxis: capillary between two Eppendorf tubes
<br/>- New test of chemotaxis: capillary between two Eppendorf tubes
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<p class="title4">Communication and financial support:</p>
<p class="title4">Communication and financial support:</p>
<p class="texte">
<p class="texte">
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- Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
+
- Support of two French city halls: Mairie de Montréal and Mairie de Ventenac en Minervois.
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<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
+
<br/>- First interview by the French national radio France Inter which will be aired on October 19<sup>th</sup>.
<br/>- Beginning of the organization of the Toulouse iGEM team acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
<br/>- Beginning of the organization of the Toulouse iGEM team acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
<br/>- Final design of the wiki
<br/>- Final design of the wiki
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<p class="title4">Lab work:</p>
<p class="title4">Lab work:</p>
<p class="texte">
<p class="texte">
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- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1-D4E1)
+
- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1 - D4E1)
<br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)
<br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)
<br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)
<br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)
<br/>- Chemotaxis test with wild type <i>B. subtilis</i> strain using Imperial College protocol
<br/>- Chemotaxis test with wild type <i>B. subtilis</i> strain using Imperial College protocol
-
<br/>- Tansformation of BBa_K1364011 + BBa_K823022 (pSBBS4S) in <i>B. subtilis</i>
+
<br/>- Tansformation of BBa_K1364011 + BBa_K823022 (pSB<sub>BS</sub>4S) in <i>B. subtilis</i>
<br/>- Culture of fungi with fungicides
<br/>- Culture of fungi with fungicides
<br/>- Sequencing of plasmids containing the fungicides
<br/>- Sequencing of plasmids containing the fungicides
</p>
</p>
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<p class="title3">Weekly meeting with the instructors(09/18/2014)</p>  
+
<p class="title3">Weekly meeting with the instructors (09/18/2014)</p>  
<p class="texte">
<p class="texte">
- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).
- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).
<br/>- The next step is to sequence the maxi operon (EcAMP1 – GAFP1 – D4E1).
<br/>- The next step is to sequence the maxi operon (EcAMP1 – GAFP1 – D4E1).
<br/>- Try different approaches for the chemotaxis:
<br/>- Try different approaches for the chemotaxis:
-
<br/>- New protocol with the capillary assay with a new system made by a glassblower.
+
<br/>New protocol with the capillary assay with a new system made by a glassblower.
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<br/>- Make the Imperial College test again by testing 3 different solutions: chitin, N-acetylglucosamine and another carbon source.
+
<br/>Make the Imperial College test again by testing 3 different solutions: chitin, N-acetylglucosamine and another carbon source.
<br/>- Decrease the number of conidia and temperature for the fungicide test.
<br/>- Decrease the number of conidia and temperature for the fungicide test.
</p>
</p>
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</div>
</div>
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<div class="technology2"><p class="title2"; "line-height:6px"; "padding:10px";>Week 15 (22 - 28 September)</div></p>
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<div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 15 (22 - 28 September)</div></p>
<div class="thelanguage2">
<div class="thelanguage2">
<p class="title4">Communication and financial support:</p>
<p class="title4">Communication and financial support:</p>
<p class="texte">
<p class="texte">
-
- New support of Sallèles d’Aude, Ventenac en Minervois, Labastide d'Anjou, Montréal city halls but also the Département de l'Hérault.
+
- New support of Sallèles d’Aude and Labastide d'Anjou city halls but also the Département de l'Hérault.
<br/>- Payment of the plane tickets.
<br/>- Payment of the plane tickets.
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<br/>- Acknowledgement day (4th December): contact with catering service, reservation of the amphitheater, list of guests…
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<br/>- Acknowledgement day (4<sup>th</sup> December): contact with catering service, reservation of the amphitheater, list of guests…
<br/>- Order of the T shirt for the Jamboree! We look awesome with them!
<br/>- Order of the T shirt for the Jamboree! We look awesome with them!
</p>
</p>
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<p class="title2">Main activites</p>
<p class="title2">Main activites</p>
<p class="texte">
<p class="texte">
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- Preparation of the wiki every day... all day long...so hard to work on it, especially at night!
+
- Preparation of the wiki every day... all day long... so hard to work on it, especially at night!
<br/>- Preparation of the poster and the presentation for the Giant Jamboree with our instructors
<br/>- Preparation of the poster and the presentation for the Giant Jamboree with our instructors
<br/>
<br/>

Revision as of 21:16, 17 October 2014