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Team:Uppsala
From 2014.igem.org
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<tr><td></td><td><h2>What we have created</h2></td></tr> | <tr><td></td><td><h2>What we have created</h2></td></tr> | ||
<tr><td><img class="schedule" src="https://static.igem.org/mediawiki/2014/e/ea/Sum_up_Uppsala14.png"</img></td> | <tr><td><img class="schedule" src="https://static.igem.org/mediawiki/2014/e/ea/Sum_up_Uppsala14.png"</img></td> | ||
| - | <td><p>Our creation, the Bactissile, is a missile bacteria, containing a two-mode system. Initially, the Bactissile will be in the <b>Target mode</b>, in which it will be producing a lot of the mobility protein, CheZ and a silencing sRNA, which will silence the expression of the Bactissiles weapon, colicin Fy. In this state, the Bactissile will be taking big leaps, randomly searching for its target, <i>Y. enterocolitica</i>. <br><br>When <i>Y. enterocolitica</i> enters the vicinity of the Bactissile, the Bactissile will switch to its <b>Attacking mode</b>. In this state, the Bactissile will stop the production of CheZ and the sRNA and initiate the production of its weapon, colicin Fy. The Bactissile will | + | <td><p>Our creation, the Bactissile, is a missile bacteria, containing a two-mode system. Initially, the Bactissile will be in the <b>Target mode</b>, in which it will be producing a lot of the mobility protein, CheZ and a silencing sRNA, which will silence the expression of the Bactissiles weapon, colicin Fy. In this state, the Bactissile will be taking big leaps, randomly searching for its target, <i>Y. enterocolitica</i>. <br><br>When <i>Y. enterocolitica</i> enters the vicinity of the Bactissile, the Bactissile will switch to its <b>Attacking mode</b>. In this state, the Bactissile will stop the production of CheZ and the sRNA and initiate the production of its weapon, the bacteriocin colicin Fy. The Bactissile will start tumbling close to the target, emitting its weapon. When the concentration of colicin Fy reaches a threshold value, the dosage will be lethal for <i>Y. enterocolitica</i>. Colicin Fy is only harmful for <i>Y. enterocolitica</i>, which entails that the beneficial gut flora in the Bactissile's surroundings will remain unharmed.</p></td></tr></table> |
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| - | <p><br><i>Figure 2. The picture shows the result from an experiment to test the killing efficiency of | + | <p><br><i>Figure 2. The picture shows the result from an experiment to test the killing efficiency of colicin Fy. On the left Y. enterocolitica has been grown in liquid culture together with colicin Fy. The right plate is a negative control, where Y. enterocolitica has grown in the same conditions in liquid culture without any colicin Fy added.</p> |
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| - | <p>We managed to produce the bacteriocin colicin Fy. By fusing it with a His-tag we could perform an SDS-page gel and prove its presence. To analyse the colicin Fy’s killing efficiency, we let <i>Y. enterocolita</i> grow with and without colicin Fy in liquid cultures and compared the result. Fig. 2 shows these two cultures plated. On the left plate, the <i>Y. enterocolitica</i> had grown with the colicin Fy added, while the right plate is the | + | <p>We managed to produce the bacteriocin colicin Fy. By fusing it with a His-tag we could perform an SDS-page gel and prove its presence. To analyse the colicin Fy’s killing efficiency, we let <i>Y. enterocolita</i> grow with and without colicin Fy in liquid cultures and compared the result. Fig. 2 shows these two cultures plated. On the left plate, the <i>Y. enterocolitica</i> had grown with the colicin Fy added, while the right plate is the negative control, where <i>Y. enterocolitica</i> had grown without any colicin Fy. If we compare the two plates, we can see that there is a clear difference in the amount of <i>Y. enterocolitica</i> colonies. |
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Revision as of 20:57, 17 October 2014
Home
Bactissiles: The future of microbial combat
Destabilized ecosystems and disturbed gut floras are both consequences of treatments that lack selectivity. More efficient and precise methods are needed. This year we, the Uppsala iGEM team, try to widen the view and find new possibilities with engineered bacteria. By developing a system that homes towards a target and secretes an affectant, we can ensure a specific outcome. Such a system could have applications in a number of different fields, though we have chosen to put this into practice in a pinpointing pathogen-killing approach. In our prototype system, introduced in E. coli , we hijack the quorum sensing system of the gut pathogen Yersinia enterocolitica . Our bacteria will be able to sense the presence of the pathogen, accumulate in its vicinity and emit a target-specific bacteriocin, leaving the remaining gut flora intact. The era of mass destruction is over. Welcome the missile bacteria, the Bactissile!
What we have created | |
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Our creation, the Bactissile, is a missile bacteria, containing a two-mode system. Initially, the Bactissile will be in the Target mode, in which it will be producing a lot of the mobility protein, CheZ and a silencing sRNA, which will silence the expression of the Bactissiles weapon, colicin Fy. In this state, the Bactissile will be taking big leaps, randomly searching for its target, Y. enterocolitica. |
Main Result
The Sensing System
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By constructing the measurement construct BBa_K1381008 (yenbox_WT-B0032-GFP) and performing double transformation together with one of the constructs producting the activator YenR BBa_K1381005 (J23110-B0034-YenR), BBa_K1381006 (J23102-B0034-YenR) and BBa_K1381007 (J23101-B0034-YenR). We managed to show that the activator YenR works perfectly fine in E. coli and that it recognise the recognition region, the yenbox and induces the strength of the promoter fused with it. By measuring the production of the green fluorescence protein GFP using a flow cytometer, we could see that we got a five-fold induction when YenR with the strongest promoter out of the three used were present.
Read more about the Sensing System
The Targeting System
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We managed to restore chemotaxis in non-motile mutant strains, by reintroducing the cheZ gene on plasmids into the cheZ-knockout E. coli. Three promoters of different strengths were tested in combination with our construct and were shown to induce different levels of motility.
Read more about the Targeting System
The Killing System
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We managed to produce the bacteriocin colicin Fy. By fusing it with a His-tag we could perform an SDS-page gel and prove its presence. To analyse the colicin Fy’s killing efficiency, we let Y. enterocolita grow with and without colicin Fy in liquid cultures and compared the result. Fig. 2 shows these two cultures plated. On the left plate, the Y. enterocolitica had grown with the colicin Fy added, while the right plate is the negative control, where Y. enterocolitica had grown without any colicin Fy. If we compare the two plates, we can see that there is a clear difference in the amount of Y. enterocolitica colonies.
Read more about the Killing System
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