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Team:Heidelberg/pages/Collaborations
From 2014.igem.org
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| + | Besides spending a whole summer in the lab and developing an own project, iGEM is about meeting new people, people with the same interests, the same motivation, the same dedication. Collaborations between team ensure the exchange of ideas, therefore helping the field of Synthetic Biology to be interdisciplinary and creative - the best conditions to give rise to revolutionary projects. We are very happy to met some of the team members from Freiburg, Aachen, Tuebingen, Marburg and London. | ||
=iGEM Team Aachen= | =iGEM Team Aachen= | ||
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=iGEM Team Tuebingen= | =iGEM Team Tuebingen= | ||
| + | The project of iGEM Team Tuebingen is about blood types and the difficulties that arises during blood donation of the wrong blood type. They aim to develop an easy system to convert blood types A, B, AB to the rares but also most applicable blood type 0. They have applied the method of intein targeting, a method which can also be found in our intein toolbox, to the N-Acetyl-Galactosaminidase protein. More about their project can be read in the very nice [http://igem14-heidelberg.tumblr.com/post/98138990630/igem-team-tubingen article] they have wrote for our tumblr blog or on their own [Team:Tuebingen wiki]. | ||
| + | In this collaboration, Tuebingen conducted a mass spectrometry analysis of our Lambda lysozyme to proof circularisation. The data still has to be evaluated, but we will have them ready until the jamboree. There is a high demand for our expression vectors. Tuebingen had problems with expressing their constructs, therefore we send them some of our plasmids pSBX1K30 ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1362093 BBa_K1362093]) and pSBX4K50 ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1362097 BBa_K1362097]), as they showed to work really nicely even with big proteins like the [Team:Heidelberg/Project/PCR2.0 Dnmt1] with 105kDa. | ||
=iGEM Team Freiburg= | =iGEM Team Freiburg= | ||
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Three of our team members, Anna, Caro, and Charlotte visited the iGEM team Freiburg. We met the team right after a big team meeting, therefore we were able to hear their latest project developments. We spend the evening talking about experiences in the lab and about iGEM. It was relieving to hear that they faced similar difficulties in the lab. | Three of our team members, Anna, Caro, and Charlotte visited the iGEM team Freiburg. We met the team right after a big team meeting, therefore we were able to hear their latest project developments. We spend the evening talking about experiences in the lab and about iGEM. It was relieving to hear that they faced similar difficulties in the lab. | ||
| - | The morning after we presented our project to the iGEM team Freiburg. Freiburg was very interested in our program iGEM@home as we offered them to promote their own human | + | The morning after we presented our project to the iGEM team Freiburg. Freiburg was very interested in our program iGEM@home as we offered them to promote their own human practice projects. You can also find an [http://igem14-heidelberg.tumblr.com/post/99936805780/optogenetics-the-lock-and-key-for-daily-life article] about optogenetics on our tumblr blog. |
Both our teams are working with light inducible systems, manly the LOV domain. Although working in different organisms, the constructs were compatible, therefore we could interchange protocols for induction and preliminary results. Furthermore we gazed at their professional light induction box - we can definitely improved ours. | Both our teams are working with light inducible systems, manly the LOV domain. Although working in different organisms, the constructs were compatible, therefore we could interchange protocols for induction and preliminary results. Furthermore we gazed at their professional light induction box - we can definitely improved ours. | ||
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After cooperation with the iGEM Team Freiburg for two years in a row we are hoping to keep Freiburg as a reliable partner in the next years as well. | After cooperation with the iGEM Team Freiburg for two years in a row we are hoping to keep Freiburg as a reliable partner in the next years as well. | ||
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Revision as of 21:36, 17 October 2014
Besides spending a whole summer in the lab and developing an own project, iGEM is about meeting new people, people with the same interests, the same motivation, the same dedication. Collaborations between team ensure the exchange of ideas, therefore helping the field of Synthetic Biology to be interdisciplinary and creative - the best conditions to give rise to revolutionary projects. We are very happy to met some of the team members from Freiburg, Aachen, Tuebingen, Marburg and London.
iGEM Team Aachen
Michael from the iGEM team Aachen visited us in Heidelberg. Their team worked on the development of a real-time pathogen detection technique for what they have build their own fluorescence measurement camera. We provided our expression vectors to the iGEM team Aachen, which they used to express their part [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1319003 E1010-K1319003], the human galectin-3 protein fused to mRFP. Nicely seen in Figure 1 and 2 the the intensity of the red color is significantly higher for the induced sample with [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1362091 pSBX1A30] compared to the uninduced ones. To ensure the right molecular mass of the fusion protein, a SDS gel was run as well (Figure 3).
More information about their project can be found on Aachens Galectin-3 site.
In exchange they offered to characterise these expression vectors with their own designed analyser by measuring the amount of produced fluorescent proteins. Unfortunately, transporting E.colis to another city wasn't as easy as expected, not all Bacteria were able to grow afterwards and we could only obtain sufficient data from pSBX4C5.
iGEM Team Tuebingen
The project of iGEM Team Tuebingen is about blood types and the difficulties that arises during blood donation of the wrong blood type. They aim to develop an easy system to convert blood types A, B, AB to the rares but also most applicable blood type 0. They have applied the method of intein targeting, a method which can also be found in our intein toolbox, to the N-Acetyl-Galactosaminidase protein. More about their project can be read in the very nice [http://igem14-heidelberg.tumblr.com/post/98138990630/igem-team-tubingen article] they have wrote for our tumblr blog or on their own [Team:Tuebingen wiki].
In this collaboration, Tuebingen conducted a mass spectrometry analysis of our Lambda lysozyme to proof circularisation. The data still has to be evaluated, but we will have them ready until the jamboree. There is a high demand for our expression vectors. Tuebingen had problems with expressing their constructs, therefore we send them some of our plasmids pSBX1K30 ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1362093 BBa_K1362093]) and pSBX4K50 ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1362097 BBa_K1362097]), as they showed to work really nicely even with big proteins like the [Team:Heidelberg/Project/PCR2.0 Dnmt1] with 105kDa.
iGEM Team Freiburg
Three of our team members, Anna, Caro, and Charlotte visited the iGEM team Freiburg. We met the team right after a big team meeting, therefore we were able to hear their latest project developments. We spend the evening talking about experiences in the lab and about iGEM. It was relieving to hear that they faced similar difficulties in the lab. The morning after we presented our project to the iGEM team Freiburg. Freiburg was very interested in our program iGEM@home as we offered them to promote their own human practice projects. You can also find an [http://igem14-heidelberg.tumblr.com/post/99936805780/optogenetics-the-lock-and-key-for-daily-life article] about optogenetics on our tumblr blog. Both our teams are working with light inducible systems, manly the LOV domain. Although working in different organisms, the constructs were compatible, therefore we could interchange protocols for induction and preliminary results. Furthermore we gazed at their professional light induction box - we can definitely improved ours.
After cooperation with the iGEM Team Freiburg for two years in a row we are hoping to keep Freiburg as a reliable partner in the next years as well.
