Team:Groningen/Template/MODULE/Notebook/vector/week1

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In silico preparation of primers for the Gibson assembly between signal sequence USP45 and modified version of Aiia.
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<b>goal</b>: Isolate <i>mrfp</i> insert out of pSB1C3 and ligate in pIL253 and pNZ8048g to make biobrick compatible vectors for <i>L. lactis</i>
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In silico production of synthetic gene ssUSP45DspB.
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<i>mrfp</i> was primed out of pSB1C3 by primers designed for pIL253 and pNZ8048g
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General preparation of lab necessities.
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The reactions were loaded on a gel and this yielded positive results for the constructs for both pIL253 and pNZ8048g
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The backbones pIL253 and pNZ8048g are digested so that the <i>mrfp</i> constructs will fit in them
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Also the <i>mrfp</i> inserts are digested with the same or complementary enzymes
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The backbones and <i>mrfp</i> inserts are checked on a gel
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The gel did not yield good results for the pNZ <i>mrfp</i>
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Revision as of 20:04, 17 October 2014

September 29 - October 5
 
goal: Isolate mrfp insert out of pSB1C3 and ligate in pIL253 and pNZ8048g to make biobrick compatible vectors for L. lactis
 
mrfp was primed out of pSB1C3 by primers designed for pIL253 and pNZ8048g
 
The reactions were loaded on a gel and this yielded positive results for the constructs for both pIL253 and pNZ8048g
 
The backbones pIL253 and pNZ8048g are digested so that the mrfp constructs will fit in them
 
Also the mrfp inserts are digested with the same or complementary enzymes
 
The backbones and mrfp inserts are checked on a gel
 
The gel did not yield good results for the pNZ mrfp
 
 
 
 

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