Team:Austin Texas/photocage
From 2014.igem.org
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After this exposure, the cells were grown for an additional 16 hours, allowing the newly decaged T7 RNAP to transcribe the GFP reporter gene, which ultimately results in fluorescence. As can be seen in '''Figure 3''', while the fluorescence background at time zero is not as low as we would like, there is a clear and dramatic increase in fluorescence that is directly dependent on the amount of time the culture was exposed to 365 nm light. This result is consistent with previous results using this system (Chou et al. 2010). | After this exposure, the cells were grown for an additional 16 hours, allowing the newly decaged T7 RNAP to transcribe the GFP reporter gene, which ultimately results in fluorescence. As can be seen in '''Figure 3''', while the fluorescence background at time zero is not as low as we would like, there is a clear and dramatic increase in fluorescence that is directly dependent on the amount of time the culture was exposed to 365 nm light. This result is consistent with previous results using this system (Chou et al. 2010). | ||
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<h1>Discussion</h1> | <h1>Discussion</h1> |
Revision as of 20:26, 17 October 2014
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