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Team:Groningen/Template/MODULE/projects/parts/detection
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We want <i>Lactococcus lactis</i> to start producing growth inhibiting molecules only when a pathogen is present. Therefore, <i>L. lactis</i> must be able to sense <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. | We want <i>Lactococcus lactis</i> to start producing growth inhibiting molecules only when a pathogen is present. Therefore, <i>L. lactis</i> must be able to sense <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>. | ||
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Revision as of 19:26, 17 October 2014
Detection system
We want Lactococcus lactis to start producing growth inhibiting molecules only when a pathogen is present. Therefore, L. lactis must be able to sense Pseudomonas aeruginosa and Staphylococcus aureus.
Detecting Pseudomonas aeruginosa
To give Lactococcus lactis the ability to detect P. aeruginosa we assembled a new BioBrick. For the detection we adopted a widely used principle for detection, namely detection of quorum molecules. P. aeruginosaproduces AHLs for communication1, therefore we have build an AHL sensor) that can be used by L. lactis.
To test the how well Lactococcus lactis can detect P. aeruginosa we build a construct which has the AHL sensor ability coupled with a GFP gene.
A related part that was made is the AHL generator. Many iGEM teams are working with AHL sensing, either to detect P. aeruginosa or for an regulatory mechanisms. Our AHL generator produces AHLs, which enables other teams to produce AHLs and test their sensors without the hassle of working with the pathogenic bacterium itself.
