Team:TCU Taiwan/Achievements

From 2014.igem.org

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   <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38">Silver</font><br>
   <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38">Silver</font><br>
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<div class="wrapper" style="background-color: white;"><p><font face="Trebuchet MS" size="5" color="#90B849">MATLAB</font><br><br>
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  <ul>
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  <p class="qbody" align="justify">&nbsp;</p>
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    <li>Experimentally validate Biobricks of our team design and  construct were as expected.</li>
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    <ul>
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      -<a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign">Construct  new system designs</a><br>
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        a. CRISPR  Construction (Figure.1)<br>
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        b. Recombinant  Phagemid Construction (Figure.2)
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    </ul>
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    <li>We have documented and  characterized of our <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts">parts</a>  in the &rdquo;Main Page&rdquo; section and registry entry.</li>
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    <ul>
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      <p>-Tests the BioBrick<br>
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        a. <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1473005">BBa_K1473005</a><br>
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        b. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473002</a><br>
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        c. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473003</a><br>
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        d. <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts ">BBa_K1473004</a></p>
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    </ul>
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    <li>Submit our new parts to iGEM Parts registry
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 <li>Because we use phages in our projct, it could cause some safety problems if phages remain in environment. So we decided to use helper phage and phagemid for phage display, this will solve these problems because those phages cannot have next generation.
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  </ul>
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   <p>&nbsp;</p>
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    <li>We improve the function of BBa_K1218011  from 2013 <a href="http://parts.igem.org/Part:BBa_K1218011">Stanford-Brown</a> .</li>
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  </ul>
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  <ul>
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    <li>Cooperation <a href="https://2014.igem.org/Team:NCTU_Formosa/human_practice ">with NCTU-Formosa</a> Team on  modeling. We provide the data to NCTU-Formosa Team then they make the model for  us.</li>
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    <li>In  our project, we still need antibiotics to reduce the amount of bacteria. So we  come up with an idea : use 16S rRNA as CIRSPR's target to avoid co-effect of  antibiotic use. We have put it in our future work. </li>
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  </ul>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" style="height: 40px; float:inherit; margin-right: 5px;"></a>Human Practice</font><br>
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<div class="wrapper" style="background-color: white;">
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    <li>
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      Held an iGEM speech at National Hualien  Girl&rsquo;s Senior High School,  the content includes  introduction of  synthetic biology, our project. We have also designed a game about basic of our gRNA.<br>
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        <li>Join the 2014 iGEM Conference in National Chiao Tung University in summer vacation, we have social contacts with <a href="https://2014.igem.org/Team:NCTU_Formosa/human_practice#Team_Exchange">several iGEM teams from China and  Taiwan</a><br>
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        <li>Share Project and Promote Synthetic Biology in Tzu Chi University <br>
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        <li>Question Survey about synthetic biology.<br>
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        <li>Promotion of iGEM and Synthetic Biology to  populace.<br>
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        <li>Face Page : <a href="https://www.facebook.com/TCUiGEM">Tzu Chi University iGEM</a>  <br>
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        <li>Skype with <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics">NCTU-Formosa</a> and <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics">NYMU-Taipei</a>
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    </li>
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  </ul>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><img src="https://static.igem.org/mediawiki/2014/3/3f/TCU-Modeling-logo.png" style="height: 40px; float:inherit; margin-right: 5px;">Computer Modeling</font><br>
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      find the best release amount of phage after helper  phage infecting.<br>
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        <li>To know under which condition can get the best infection  rate of our phagemid-carrying phage.
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    </li>
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  </ul>
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  <legend class="qnum" align="center"><font face="Trebuchet MS" size="6" color="#A66B38"><img src="https://static.igem.org/mediawiki/2014/2/21/TCU-Safety-logo.png" alt="" style="height: 40px; float:inherit; margin-right: 5px;">Safety</font><br>
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      <p>In our experiment, we choose  M13 phage as model. This filamentous phage will make its genome a double strand  plasmid in host bacteria&rsquo;s cytosol. Therefore, M13 phage is not either a lytic  or lysogenic phage, its release will not cause host cell&rsquo;s death. </p>
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Revision as of 19:49, 17 October 2014



 
Achievements
  
 
Bronze
  • Form a team.
  • Complete judging form.
  • Constructed attractive Wiki page.
  • Present a poster and a talk were readied for Giant Jamboree.
  • Several Biobricks were built, improved and documented by our team.

 
 
Silver
  • Experimentally validate Biobricks of our team design and construct were as expected.
  • We have documented and characterized of our parts in the ”Main Page” section and registry entry.
  • Submit our new parts to iGEM Parts registry 
  • Because we use phages in our projct, it could cause some safety problems if phages remain in environment. So we decided to use helper phage and phagemid for phage display, this will solve these problems because those phages cannot have next generation.

 
Gold
  • Cooperation with NCTU-Formosa Team on modeling. We provide the data to NCTU-Formosa Team then they make the model for us.
  • In our project, we still need antibiotics to reduce the amount of bacteria. So we come up with an idea : use 16S rRNA as CIRSPR's target to avoid co-effect of antibiotic use. We have put it in our future work.
 
Human Practice
  • Held an iGEM speech at National Hualien Girl’s Senior High School, the content includes introduction of synthetic biology, our project. We have also designed a game about basic of our gRNA.
  • Join the 2014 iGEM Conference in National Chiao Tung University in summer vacation, we have social contacts with several iGEM teams from China and Taiwan
  • Share Project and Promote Synthetic Biology in Tzu Chi University
  • Question Survey about synthetic biology.
  • Promotion of iGEM and Synthetic Biology to populace.
  • Face Page : Tzu Chi University iGEM
  • Skype with NCTU-Formosa and NYMU-Taipei
 
Computer Modeling
  • find the best release amount of phage after helper phage infecting.
  • To know under which condition can get the best infection rate of our phagemid-carrying phage.
 
Safety

  • In our experiment, we choose M13 phage as model. This filamentous phage will make its genome a double strand plasmid in host bacteria’s cytosol. Therefore, M13 phage is not either a lytic or lysogenic phage, its release will not cause host cell’s death.

 
 
^


    
Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

Lost the way? Use it to help you if you're lost.

^

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