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Team:Goettingen/proLP
From 2014.igem.org
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<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#E_cra"><i>E.coli</i> cracking</a></li> | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Auto_act">Auto-activity Test</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot"> | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_robot">Yeast-Two-Hybrid Assay with Robot</a></li> |
| + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony#Y2H_screen">Yeast-Two-Hybrid Screening</a></li> | ||
</ul> | </ul> | ||
<h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li></h4> | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_protein">Protein Assessment</a></li></h4> | ||
Latest revision as of 21:17, 17 October 2014
Overview
PCR Methods
Plasmid Construction
- Restriction of DNA
- Ligation of DNA fragments
- BP recombination reaction
- LR recombination reaction
- SEAMLESS Cloning
- Peptide Library construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
