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Team:Nankai/Characterization
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Revision as of 19:19, 17 October 2014
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What we have to do is insert the genes rhlABRI to the plasmid pBBR1MCS-2. To accomplice this, we figure out 4 steps as followed.
1.Restriction endonuclease site
Considering P. aeruginosa SQ6 is a wild-type strain, we retained the genes’ native promoter from the strain. Comparing the restriction sites of multiple cloning sites of the plasmid pBBR1MCS-2 and the genes rhlABRI including the promoter, we chose the restriction endonuclease sites, Hind III and Sal I.
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